Cattle LIF(Leukemia Inhibitory Factor) ELISA Kit
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Catalog numberELK7525
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PricePlease ask
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Size96T
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Alternative NamesCDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin
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Assay TypeSandwich
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Sensitivity12.9pg/mL
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Standard2000pg/mL
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Detection range31.2-2000pg/mL
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Assay length3h
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Research AreaCytokine;Tumor immunity;Infection immunity;Neuro science;Bone metabolism;
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Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukemia Inhibitory Factor (LIF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukemia Inhibitory Factor (LIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukemia Inhibitory Factor (LIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leukemia Inhibitory Factor (LIF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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DescriptionAplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Gene target
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Gene symbolLIF-AS1, LIF-AS2, LIF
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Short nameCattle LIF(Leukemia Inhibitory Factor) ELISA Kit
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesCattle
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Alternative nameCattle leukemia inhibitory factor(Leukemia Inhibitory Factor) Enzyme-linked immunosorbent assay reagent
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Alternative techniqueelisas, kits
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Alternative to gene targetleukemia inhibitory factor, CDF and DIA and HILDA and MLPLI, LIF and IDBG-3968 and ENSG00000128342 and 3976, growth factor activity, Extracellular, Lif and IDBG-142317 and ENSMUSG00000034394 and 16878, LIF and IDBG-636335 and ENSBTAG00000007424 and 280840
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Virusleukemia
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Gene info
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Identity
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Gene
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Long gene nameLIF antisense RNA 1
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Synonyms
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Locus
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Discovery year2017-04-28
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Pubmed identfication
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene nameLIF antisense RNA 2
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Locus
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Discovery year2020-10-07
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene nameLIF interleukin 6 family cytokine
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Synonyms gene name
- leukemia inhibitory factor
- LIF, interleukin 6 family cytokine
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Synonyms
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Synonyms name
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Locus
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Discovery year1989-06-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- Interleukin 6 type cytokine family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.
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Tree numbers
- C04.557.337.372
- C04.619.531
- E05.598.500.496.500
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Qualifiersdiagnosis, parasitology, immunology, microbiology, diagnostic imaging, physiopathology, rehabilitation, enzymology, etiology, mortality, epidemiology, blood, cerebrospinal fluid, chemically induced, congenital, diet therapy, drug therapy, therapy, psychology, urine, virology, history, classification, economics, embryology, genetics, nursing, radiotherapy, ethnology, prevention & control, pathology, metabolism, complications, surgery