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Synonyms
AITR, GITR, TNFRSF18, CD357
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Alternative_names
AITR, GITR, TNFRSF18, CD357
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Description
Tumor necrosis factor receptor superfamily member 18
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
≥92%
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Assay
SDS-PAGE
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Purity
≥92%
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Endotoxin Level
< 1 EU/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Human GITR/TNFRSF18, His Tag at 2 µg/mL (100 µL/well) can bind r-Human GITR Ligand /TNFSF18 with a linear range of 0.8-25 ng/mL.
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Binding Capacity
Immobilized Human GITR/TNFRSF18, His Tag at 2 µg/mL (100 µL/well) can bind r-Human GITR Ligand /TNFSF18 with a linear range of 0.8-25 ng/mL.
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Molecular Weight
This protein is fused with a 6× His tag at C-terminus and has a calculated MW of 15.4 kDa. The protein migrates as 22-25 kDa in SDS-PAGE due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml
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Background Information
Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TNFRSF18
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Short name
CellExp™ GITR / TNFRSF18, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ GITR / tumor necrosis factor receptor superfamily, member 18, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
tumor necrosis factor receptor superfamily, member 18, AITR and CD357 and GITR and GITR-D, TNFRSF18 and IDBG-84584 and ENSG00000186891 and 8784, protein binding, Extracellular, Tnfrsf18 and IDBG-208288 and ENSMUSG00000041954 and 21936, TNFRSF18 and IDBG-635325 and ENSBTAG00000015632 and 516256
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