Mouse Anti-Mouse Slo2.1 Monoclonal IgG1, Clone S11-33
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Catalog numberSMC-324D-ALP
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PricePlease ask
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Size100 µg
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CloneS11-33
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ImmunogenFusion protein amino acids 564-624 of mouse Slo2.1 (Slick)
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Antibody s full descriptionMouse Anti-Mouse Slo2.1 Monoclonal IgG1 Antibody, Clone: S11-33: Alkaline Phosphatase
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameKCNT2 Antibody, KCa4.2 Antibody, SLICK Antibody, Slo2.1 Antibody, sodium and chloride activated ATP sensitive potassium channel Slo2.1 Antibody
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Raised inMouse
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Antibody s targetSlo2.1
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Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Calcium-Activated Potassium Channels
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~140kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-324 was sufficient for detection of Slo2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextSlo2.1 is a novel member of the mammalian Slo potassium channel gene family (1). The slick channel is activated by intracellular Na+ and Cl- and is inhibited by intracellular ATP. It is also widely expressed in the CNS and detected in the heart (2).
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Bibliography1. Santi C.M., et al. (2006) J Neuroscience 26(19): 5059-5068. 2. Bhattacharjee A., et al. (2003) J Neuroscience 23(37): 11681-11691.
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Released date17-Jul-2014
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_940905.2
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Gene number343450
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Protein numberQ6UVM3
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody, Clone S11-33 (SMC-324). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody (SMC-324) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive (nuclei and cytoplasm). Western Blot analysis of Rat brain membrane lysate showing detection of Slo2.1 Potassium Channel protein using Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody, Clone S11-33 (SMC-324). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody (SMC-324) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody, Clone S11-33 (SMC-324). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody (SMC-324) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis. Mouse Anti-Slo2.1 Potassium Channel Antibody [S11-33] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-324) Mouse Anti-Slo2.1 Potassium Channel Antibody [S11-33] used in Western Blot (WB) on Rat brain membrane lysate (SMC-324) Mouse Anti-Slo2.1 Potassium Channel Antibody [S11-33] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-324)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolRPS11, SNORD115-33, IGKV1D-33, IGLV2-33, IGHV3-33, IGHVII-33-1, IGHV3-33-2, C3orf33, RNU6-33P
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Short nameMouse Anti-Mouse Slo2 1 Monoclonal IgG1, Clone S11-33
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelAlkaline Phosphatase
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Slo2.1 monoclonal IgG1, clonality S11-33
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Alternative techniquemurine, antibodies
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Clone nameS11-33
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Gene info
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Identity
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Gene
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Long gene nameribosomal protein S11
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNA protein coding host genes
- S ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-33
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-33
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 2-33 (non-functional)
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Synonyms gene name
- immunoglobulin lambda variable 2-33
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-33
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-33-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-33-1
- immunoglobulin heavy variable (II)-33-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-33-2 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-33-2
- immunoglobulin heavy variable 3-33-2 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namechromosome 3 open reading frame 33
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-01-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 33, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 33
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
