Super Growth Top Agar is a highly rich medium used to obtain high yields of lambda bacteriophage in liquid lysates. To prepre the media d issolve the powder in d istilled water, heat to boiling if it is necessary, sterilize by autoclaving at 121°C for 15 minutes, then mix well and d ispense as desired.
Appearance of powder
Cream to yellow coloured, homogeneous, free flowing powder.
Quantity required by litre
Organisms (ATCC) Growth
Escherichia coli: good-luxuriant
Colour and Clarity
Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Tryptone 35.00 Yeast extract 20.00 Sodium chloride 5.00 Agar 7.00 Final pH (at 25°C) 7.5 ± 0.2
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
Store below 30°C and the prepared medium at 2 - 8°C.
Molecular Biology Growth Media for Escherichia coli
Super Growth Top Agar
Super Growth Top media gelling agent