CD1E, human recombinant

• Catalog number
  7308-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  T-cell surface glycoprotein CD1e, CD1A, R2
• Alternative_names
  T-cell surface glycoprotein CD1e, CD1A, R2
• Description
  Mediates the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥85%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 microgram of protein
• Molecular Weight
  33.1 kDa (297 aa, 32-305 aa + His tag), confirmed by MALDI-TOF.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
• Background Information
  CD1E is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. CD1E localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members. Recombinant human CD1E protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSEEQLSFR MLQTSSFANH SWAHSEGSGW LGDLQTHGWD TVLGTIRFLK PWSHGNFSKQ ELKNLQSLFQ LYFHSFIQIV QASAGQFQLE YPFEIQILAG CRMNAPQIFL NMAYQGSDFL SFQGISWEPS PGAGIRAQNI CKVLNRYLDI KEILQSLLGH TCPRFLAGLM EAGESELKRK VKPEAWLSCG PSPGPGRLQL VCHVSGFYPK PVWVMWMRGE QEQRGTQRGD VLPNADETWY LRATLDVAAG EAAGLSCRVK HSSLGGHDLI IHWGGYS
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CD1E  
• Gene symbol
  CD1E
• Short name
  CD1E, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  CD1e molecule, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  CD1e molecule, CD1A and R2, CD1E and IDBG-103747 and ENSG00000158488 and 913, lipid binding, Plasma membranes, BT.49600 and IDBG-630922 and ENSBTAG00000009421 and 510832

Gene info

• Identity
  HGNC:1638
• Gene
  CD1E
• Long gene name
  CD1e molecule
• Synonyms gene name
  • CD1E antigen, e polypeptide
  • CD1e antigen
• GenBank acession
  • AJ289111
• Locus
  1q23.1
• Discovery year
  1990-06-11
• Entrez gene record
  913
• Pubmed identfication
  • 10948205
• RefSeq identity
  • NM_030893
• Classification
  • C1-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000017515

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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