Mouse Monoclonal IgG1 Anti-Alpha-DEFENSIN (1-3), HNP1-3 [DEF-3]
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Catalog numberHNP103
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PricePlease ask
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Size100 µg
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AvailabilityPlease contact us to check the availability
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ImmunogenNative Defensins
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SpecificityHuman Neutrophil Peptides (HNP1-3) syn. alpha-Defensins 1-3, reactivity with HNP-4 not tested
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Species reactivityHuman, cross-reactivity with rhesus monkey and primates expected
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Product presentationAffinity purified IgG, lyophilized from PBS, BSA, and sodium azide**, reconstitute with 0.5 ml sterile water to get a stock solution of 200 µg/ml.
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG1
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Antibody s cloneDEF-3
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Product is supplied aslyophilisate
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Recommended concentration for use0.2 µg/ml IHC(C), 0.5 µg/ml IHC(P)
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Protein concentration200 µg/ml IgG after reconstitution with 0,5 ml distilled water
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Positive controlInfected skin or oral mucosa
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PretreatmentFormaldehyde-fixed tissue requires antigen unmasking preferred methods are 0.1% Pronase, Proteinase K or Trypsin.
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Tested applicationsIHC(C, P), FACS
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Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage methodStore at -20'°C'
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UniProt numberGo to UniProt/SwissProt
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Litterature1. Ganz T. et al. (1985) J. Clin. Invest., 76; 1427 ff 2. Ganz T., et al. (1992) Med. Microbiol. Immunol. 181; 99ff 3. Mizukawa N ; Sugiyama K ; Fukunaga J ; Ueno T ; Mishima K ; Takagi S ; Sugahara T (1998) Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma patients. Anticancer Res 18(6B):4645-4649. 4. Linzmeier R., Ho C.H., Hoang B.V. , and Ganz T. (1999) A 450 kb contig of defensin genes on human chromosome 8p23. Gene 233; 205-211. 5. Mikuzawa N. Sugiyama K., Kamio M., Yamachika E., Ueno T., Fukunaga J., Takagi S., Sugahara T. (2000) Immunohistochemical staining of human alpha-defensin-1 (HNP-1) in submandibular glands of patients with oral carcinomas. Anticancer Res. 20(2B); 1125-1127.
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TipsGentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThe Research is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolDEFA3, RBM6, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12, MIR9-3
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Short nameResearch
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TechniqueMouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG1
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SpeciesMouse, Mouses
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Alternative nameAlpha-DEFENSIN (1-3), HNP1-3
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene namedefensin alpha 3
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Synonyms gene
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Synonyms gene name
- defensin, alpha 3, neutrophil-specific
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Defensins, alpha
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA binding motif protein 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-09-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RNA binding motif containing
- G-patch domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 14
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 12
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namemicroRNA 9-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR9 family
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
