TRIF Antibody
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Catalog numberR31002
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenTRIF
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC-P, IHC-F
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Recommended dilutionsWestern blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml
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NotesThe stated application concentrations are suggested starting points. Titration of the TRIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis TRIF antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ8IUC6
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PurityAntigen affinity
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DescriptionTIR Domain-containing adaptor molecule 1, also known as TICAM1 or TRIF, is an adapter in responding to activation of toll-like receptors (TLRs). It mediates the delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al.(2003) mapped the gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al.(2003) showed that the protein interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TRIF mediates dsRNA activation of IFNB, through either NFKB, AP1, or IRF3. TRIF activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2. siRNA blockage of the protein reduced IFNB production in response to dsRNA.
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ImmunogenAn amino acid sequence from the C-terminus of human TRIF (NNHMWNQRGSQAPEDKTQEAE) was used as the immunogen for this TRIF antibody.
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StorageAfter reconstitution, the TRIF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolTRIM69, TICAM1
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Short nameAnti-TRIF
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to TRIF
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nametripartite motif containing 69
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Synonyms gene
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Synonyms gene name
- ring finger protein 36
- tripartite motif-containing 69
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-01-10
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Classification
- Ring finger proteins
- Tripartite motif containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametoll like receptor adaptor molecule 1
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Synonyms gene name
- toll-like receptor adaptor molecule 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-06-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- TIR domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
