Donkey anti Goat IgG H + L Alk Phos

Category

Secondary Antibody
Antibody Subtype

Conjugated Secondary Antibodies
Area of research

Immunology
Type of Immunogen

Donkey anti-goat IgG (H + L) (Alk Phos) was raised in donkey using goat IgG (H & L) ..
Raised in

Donkey
Specificity

Goat
Cross Reactivity

Should be determined by the end user
Conjugate Tag

Alkaline Phosphatase
Method of Purification

Donkey anti Goat IgG (H + L) (Alk Phos) was purified by affinity chromatography.
Form Buffer

Lyophilized from 0.01M Na3PO4, 0.25 M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.
Storage

Store at 4 deg C.
Shipping conditions

Blue Ice
Tested for

ELISA; WB
Usage Recommendations

ELISA: 1:5,000-1:50,000, WB: 1:5,000-1:50,000
URL
More info
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Latin name

Equus africanus asinus, Capra aegagrus hircus
About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Gene target

Alk Phos
Short name

anti Goat IgG H + L Alk Phos
Technique

anti, Goat, IgG, antibody to, goats, IgGs
Host

Goat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
Isotype

IgG, IgG
Species

Donkeys, Goats
Alternative name

Donkey anti Goat IgG (H + L) secondary antibody (Alk Phos)
Alternative technique

antibodies, caprine, immunoglobulins
Alternative to gene target

anaplastic lymphoma receptor tyrosine kinase, CD246 and NBLST3, ALK and IDBG-42084 and ENSG00000171094 and 238, transferase activity, Plasma membranes, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data