EGFP Antibody, Biotin Conjugated
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Catalog numberbs-2194R-Biotin
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Price:
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Size0.1ml
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Long nameEGFP Polyclonal Antibody, Biotin Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationBiotin
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenEGFP
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SpecificityThis is a highly specific antibody against EGFP. This antibody will work best with EGFP that has a similar amino acid make-up as that of Aequorea Victoria.
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ModificationUnmodified
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Modification siteNone
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourcePurified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria
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Tested applicationsWB, IHC-P
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500)
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CrossreactivityOthers
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Crossreactive species detailsEGFP protein
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Antigen backgroundThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation EmissionN/A
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SynonymsEnhanced Green Fluorescent Protein.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugationBiotinylated
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ConjugateThe Green Fluorescent Protein (eGFP) of Anti-EGFP can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
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French translationanticorps
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Gene target
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Short nameAnti-EGFP
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TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
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IsotypeImmunoglobulin G (IgG)
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LabelBiotin
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Alternative nameAnti-EGFP PAb Biotin
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data