EGFP Antibody, ALEXA FLUOR 488
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Catalog numberbs-2194R-A488
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Price:
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Size100ul
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Long nameEGFP Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
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Also known asAnti-EGFP PAb ALEXA FLUOR 488
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CategoryConjugated Primary Antibodies
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Conjugated withALEXA FLUOR® 488
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenEGFP
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SpecificityThis is a highly specific antibody against EGFP.
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ModificationUnmodified
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Modification SiteNone
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourcePurified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityOthers
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Cross reactive species detailsEGFP protein
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Background of the antigenThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
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PurificationPurified by Protein A.
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Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission499nm/519nm
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SynonymsEnhanced Green Fluorescent Protein.
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PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-EGFP has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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ConjugationAlexa Fluor
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ConjugateThe Green Fluorescent Protein (eGFP) of Anti-EGFP can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
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French translationanticorps
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Short nameAnti-EGFP
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 488
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Alternative nameEGFP Polyclonal Antibody, ALEXA FLUOR 488
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data