Mitochondrial DNA Purification Kit
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Catalog number
K389-25
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Price
Please ask
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Size
25 purifications
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Description
Yields highly purified mt. DNA, no genomic DNA contamination
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Summary
• Species reactivity- Mammalian • PCR, cloning, DNA hybridization, southern blotting, genotyping, restriction enzyme digest analysis, sequencing, etc.
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Species Reactivity
Mammalian
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Applications
PCR, cloning, DNA hybridization, southern blotting, genotyping, restriction enzyme digest analysis, sequencing, etc.
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Sample Type
Cell cultures (adherent and suspension), fresh and frozen tissues
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Features Benefits
• Simple procedure; takes less than 2 hours • Fast and convenient • The kit provides unique formulations of buffers, reagents anc columns for isolating a highly enriched mitochondrial fraction. This kit provides genomic-free DNA samples
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Storage Conditions
-20°C/RT
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Shipping Conditions
gel pack
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Shelf life
24 months
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Background
Mitochondria are essential double-membrane bound organelles that support aerobic respiration and most of the cellular ATP production in all nucleated mammalian cells. To date, hundreds of pathogenic mitochondrial DNA mutations have been identified to result in disease phenotypes such as cancer, ataxia, and diabetes. As a result, isolation of high quality mitochondrial DNA from mammalian cells and tissues in sufficient quantities is important for many basic research and clinical applications. BioVision’s Mitochondrial DNA Isolation Kit facilitates purification of quality mitochondrial DNA from mammalian cultured cells or tissue. This kit utilizes a density separation step to isolate mitochondria from the rest of the cellular organelles, followed by an enzymatic reaction to release DNA from the mitochondria. Upon release, mitochondrial DNA is further purified from nuclear DNA contamination and adsorbed onto a silica spin-column under chaotropic conditions, eliminating the use of toxic organic compounds or solvents. Mitochondrial DNA purified by this kit is suitable for various downstream molecular biology applications such as PCR, cloning, DNA hybridization, Southern Blotting, sequencing, genotyping, enzymatic analysis, and more.
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Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Mitochondrial DNA Purification Kit
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Technique
Purification, dna, purified
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Alternative name
Mitochondrial Desoxyribonucleic acid purification reagent
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Alternative technique
purifications, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
mitochondrial
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
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Tree numbers
- E01.370.225.812.735.830
- E05.200.812.735.830
- E05.478.566.380.810
- E05.478.566.639.810
- E05.478.594.760.830
- E05.601.470.380.810
- E05.601.470.639.810
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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