Stool DNA Purification Kit
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Catalog number
K1474-250
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Price
Please ask
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Size
250 Preps
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Description
Using spin columns
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Summary
High-quality DNA is purified from stool or fecal samples using spin columns
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Detection Method
DNA is purified from stool or fecal samples using spin columns
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Applications
PCR, restriction digestion, and hybridization techniques
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Sample Type
Stool or fecal samples
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Features Benefits
High quality spin columns,Rapid, easy and convenient,Highly pure, high yield,Many downstream applications such as PCR, restriction digestion, and hybridization techniques
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Storage Conditions
Multiple Temperatures
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Shipping Conditions
RT
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Shelf life
12 months
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Background
The Stool DNA Purification Kit is designed for the rapid and reliable purification of high-quality DNA from stool or fecal samples. Up to 0.25 gm of stool samples can be processed in less than an hour. The kit protocol does not need organic extraction and multiple samples can be processed in parallel. The kit combines the reversible nucleic acid-binding properties of spin columns with a propriety buffer system to eliminate PCR inhibiting compounds such as humic acid from stool samples. In this kit, stool samples are homogenized and then treated in a specially formulated buffer that contains a detergent. Humic acid, proteins, polysaccharides and other contaminants are subsequently precipitated after a heat-freeze step. DNA is further purified using a spin-column. A rapid wash step removes trace contaminants and pure DNA is eluted in sterile water or low ionic strength Elution Buffer. Purified DNA can be used in downstream applications such as PCR, restriction digestion, and hybridization techniques.
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Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Stool DNA Purification Kit
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Technique
Purification, dna, purified
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Alternative name
Stool Desoxyribonucleic acid purification reagent
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Alternative technique
purifications, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
Product images
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