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Summary
• Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Applications- This assay detects mouse ANGPTL3 in the range of 0.016 – 1 ng/ml ANGPTL3/ml. The lowest level of ANGPTL3 that can be detected by this assay is 15 pg/ml.
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Applications
ELISA
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Sample Type
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
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Features Benefits
• Simple procedure • Fast and convenient • High-throughput adaptable
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Storage Conditions
+4°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
The angiopoietins are a family of growth factors that are specific for vascular endothelium. The full-length cDNA encoding angiopoietin-like protein 3 (ANGPTL3) from a human fetal liver/spleen cDNA library has 460-amino acid and the characteristic structure of angiopoietins: a signal peptide, an extended helical domain predicted to form dimeric or trimeric coiled-coils, a short linker peptide, and a globular fibrinogen-like domain (FLD). Human ANGPTL3 shares 76% amino acid sequence identity with mouse Angptl3. Northern blot analysis of human tissues showed a preferential expression of 4 ANGPTL3 transcripts being 4.5, 3.0, 2.8, and 1.7 kb in liver. ANGPTL3 can induce angiogenesis in the rat corneal assay. The FLD alone was sufficient to induce endothelial cell adhesion and in vivo angiogenesis. Microarray analysis showed that mouse hematopoietic stem cell (HSC)-supportive fetal liver CD3-positive cells expressed Angptl2 and Angptl3. The ANGPTL3 ELISA Kit is to be used for the in vitro quantitative determination of mouse/ rat ANGPTL3 in biological fluids. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse or rat ANGPTL3 in biological fluids. A polyclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a biotinylated polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples. The assay range is 0.016 – 1 ng/ml ANGPTL3/ml. The lowest level of ANGPTL3 that can be detected by this assay is 15 pg/ml.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats. Serum should be stored at +5°C
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Additional description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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Gene target
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Gene symbol
ANGPTL3
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Short name
ANGPTL3 (mouse/ ) Serum ELISA Kit
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Technique
ELISA kit, ELISA, Mouse, serum, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Host
mouse, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Rat, Mouses, Rats
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Alternative name
angiopoietin-like 3 (mouse/rat) blood serum Enzyme-linked immunosorbent assay reagent
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Alternative technique
sera, murine, elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
serum
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