hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)
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Catalog numberCAS620A-KIT
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PricePlease ask
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Size1 kit
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Product categoryCas9
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Shipping TemperatureRT/Blue Ice/Dry Ice
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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DescriptionA primer is a strand of short nucleic acid sequences (generally about 10 base pairs) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand
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Gene target
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Gene symbolAAVS1, PPP1R12C, STAG1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, SPAG6, IGKV1OR2-1, MIR101-1
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Short namehspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer confirm Cas9 integration site), CAS9-PR-1 (PCR primers confirm Cas9 expression)
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Techniqueprimers, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. primer, Vectors
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Labelpuro
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Alternative namehspCas9 AAVS1 Safe Harbor Knock-in origin (AAVS1-SA-puro-EF1-hspCas9), Complete reagent including CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting integrating Desoxyribonucleic acid sequence), CAS640PR-1 (Junction PCR test kit Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR test kit primers to confirm Cas9 expression)
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Alternative techniquepcr, pcr-sequence, kits, dna-amplification
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissuedonor
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Gene info
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Identity
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Gene
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Long gene nameadeno-associated virus integration site 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-09-10
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 regulatory subunit 12C
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Synonyms gene
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Synonyms gene name
- leukocyte receptor cluster (LRC) member 3
- protein phosphatase 1, regulatory (inhibitor) subunit 12C
- protein phosphatase 1, regulatory subunit 12C
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Myosin phosphatase targeting family
- Protein phosphatase 1 regulatory subunits
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namestromal antigen 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-04-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
- Cohesin complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namesperm associated antigen 6
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-04-23
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Entrez gene record
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Pubmed identfication
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Classification
- Armadillo repeat containing
- Cilia and flagella associated
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 101-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR101 family
MeSH Data
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Name
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ConceptScope note: Techniques used to add in exogenous gene sequence such as mutated genes; REPORTER GENES, to study mechanisms of gene expression; or regulatory control sequences, to study effects of temporal changes to GENE EXPRESSION.
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Tree numbers
- E05.393.335.249
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
