AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
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Catalog numberGE622A-KIT
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PricePlease ask
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Size1 kit
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Shipping TemperatureRT/Blue Ice/ Dry Ice
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Product categoryGene Editing
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Gene symbolAAVS1, PPP1R12C, STAG1, SPAG6, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1
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Short nameAAVS1 Safe Harbor Targeting Vector 2 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) GE640PR-1 (Junction PCR Primer confirm AAVS1 integration site)
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TechniquePCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Vectors
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Labelpuro
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Alternative nameAAVS1 Safe Harbor Targeting integrating Desoxyribonucleic acid sequence 2.0 - GOI Knock-in origin (AAVS1-SA-puro-EF1-MCS), Complete reagent including CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting integrating Desoxyribonucleic acid sequence) and GE640PR-1 (Junction PCR test kit Primer Mix to confirm AAVS1 integration site)
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Alternative techniquepcr-sequence, kits, dna-amplification
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissuedonor
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Gene info
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Identity
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Gene
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Long gene nameadeno-associated virus integration site 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-09-10
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 regulatory subunit 12C
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Synonyms gene
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Synonyms gene name
- leukocyte receptor cluster (LRC) member 3
- protein phosphatase 1, regulatory (inhibitor) subunit 12C
- protein phosphatase 1, regulatory subunit 12C
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Myosin phosphatase targeting family
- Protein phosphatase 1 regulatory subunits
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namestromal antigen 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-04-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
- Cohesin complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesperm associated antigen 6
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-04-23
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Entrez gene record
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Pubmed identfication
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Classification
- Armadillo repeat containing
- Cilia and flagella associated
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
MeSH Data
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Name
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ConceptScope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
- E05.599.495