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Alternative name1
Anti-Donkey Anti-Goat IgG (H+L), Rat/Mouse ads-HRP
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Alternative name2
Donkey Anti-Caprine IgG (H+L), Rat/Murine ads-HRP
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Alternative name3
Donkey Anti-Caprinae IgG (H+L), Rattus norvegicus/Mus musculus ads-HRP
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Alternative name4
Donkey Anti-Goat IgG (H+L)
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Alternative name5
Donkey Anti-Goat IgG(H+L), Rat/Mouse ads - HRP; Donkey Anti-Goat IgG (H+L)
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Other names
N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Secondary Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
IgG
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Clone
Polyclonal antibody
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Host organism
Donkey
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Species reactivity
Goat
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Specificity and cross reactivity
Reacts with the heavy and light chains of Goat IgG as demonstrated by ELISA. May also react with the light chains of other goat immunoglobulins.
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Purification method
Affinity chromatography on Goat IgG covalently linked to agarose.
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Form Appearance
Horseradish Peroxidase (HRP) Conjugate
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Concentration
N/A
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Storage and shipping
The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at The items should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at The items should be kept in the range of 1-7 degrees Celsius. or long-term productone should be stored at -20 degrees Celsius.. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at The items should be kept in the range of 1-7 degrees Celsius. or long-term productone should be stored at -20 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at The items should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
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Tested for
ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Equus africanus asinus, Capra aegagrus hircus, Mus musculus, Rattus norvegicus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short name
Anti-Goat IgG (H+L), /Mouse ads-
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Technique
anti-, Mouse, anti, Goat, IgG, antibody to, antibodies, goats, IgGs, mouses
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Host
Goat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, IgG
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Label
HRP
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Species
Mouse, Donkeys, Goats, Mouses, Rats
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Alternative name
Donkey Antibody toGoat Immunoglobulin G (heavy + light chain), Rat/Mouse ads-horseradish peroxidase
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Alternative technique
murine, antibodies, caprine, immunoglobulins
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