Neutral Red Cell Cytotoxicity Assay Kit

• Catalog number
  K447-1000
• Price
  Please ask
• Size
  1000 assays

• Summary
  • Detection method- Absorbance (540 nm) • Sample type- Cell culture (adherent and non adherent • Species reactivity- Mammalian • Applications- This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.
• Detection Method
  Detection method- Absorbance (540 nm)
• Species Reactivity
  Species reactivity- Mammalian
• Applications
  This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.
• Sample Type
  Cell cultures (adherent and non-adherent)
• Features Benefits
  • Simple procedure; takes less than 2 hours • Fast, sensitive and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Neutral red cell cytotoxicity assay is one of the common methods used to detect cell viability or drug cytotoxicity. The principle of this assay is based on the detection of viable cells via the uptake of the dye neutral red. Neutral red is a eurhodin dye that stains lysosomes in viable cells. Viable cells can take up neutral red via active transport and incorporate the dye into their lysosomes but non-viable cells cannot not take up this chromophore. Consequently, after washing, viable cells can release the incorporated dye in under acidified-extracted conditions. The amount of released dye can be used to determine the total number of viable cells or drug cytotoxicity. The neutral red uptake assay provides a quantitative measurement of the number of viable cells and can be measured at OD 540 nm. BioVision’s Neutral Red Cell Cytotoxicity Assay Kit is simple, accurate, and reproducible. It also includes Doxorubicin, as a positive control. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5,000-50,000 cells per well.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.
• Conjugation
  red dye

• Gene target
  Neutral   Red   Cytotoxicity   Kit  
• Short name
  Neutral Red Cytotoxicity Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Label
  Red
• Alternative name
  Neutral Red cellular Cytotoxicity test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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