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Description
Neutral Red Solution is a readymade stain reported to be used for Neisser's staining procedure of metachromatic granules.
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Application
For microbiological / pathological laboratories, hospitals, research settings and educational institutes. These stains augment tissue morphology, demonstrate important cellular components, aid in the evaluation of diseases, and identifies pathogenic organisms.
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Classification
Metachromatic Stains
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Composition
Neutral red 1.000 gm Acetic acid 2.000 ml Distilled water 1000.000 ml
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Appearance
Red coloured solution.
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Clarity
Clear without any particles.
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Microscopic Examination
Metachromatic staining is carried out where Neutral red is used as counter stain and staining characteristic of organism is observed under microscope by using oil immersion lens.
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Results
Metachromatic granules : deep blue, The Organisms : pink coloured.
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Storage
Store below 30°C in tightly closed container and away from bright light.
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Shelf Life
Use before expiry date on label.
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Conjugation
red dye
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Additional description
The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
