Anti-Mouse CD4 FITC (Clone CT-CD4) (rat IgG2a)
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Catalog number
GEN520110
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Price
Please ask
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Size
0.3 miligrams
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Also known as
CD4
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Other names
CD4; CD4 protein; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4 molecule; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD4
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Gene name synonims
N/A
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Other gene names
CD4; CD4; CD4mut; CD4 receptor
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Clonality
Monoclonal
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Immunoglobulin isotype
Rat IgG2a
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Clone
CT-CD4
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Host organism
Rat (Rattus norvegicus)
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
CD4 (L3/T4; Ly4) (Mouse CD4); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
FITC
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Concentration
Antibody Concentration: 0.1 miligram per 1 mililiter
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Storage and shipping
Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles and prolonged exposure to light. Check label for expiry date.
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Tested applications
Immunohistochemistry (IHC)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
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Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbol
CD4, SLC6A10P, CTF1, PCYT1A, LUZP4
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Short name
Anti-Mouse CD4 FITC (Clone CT-CD4) ( IgG2a)
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Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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Host
mouse
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Isotype
IgG2a
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Label
FITC
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Species
Rat, Mouses, Rats
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Alternative name
Anti-Anti-Mouse CD4 FITC (Clone CT-CD4) (rat IgG2a)
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Alternative technique
murine, antibodies, fluorescine
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Alternative to gene target
CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
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Clone name
CT-CD4
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Gene info
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Identity
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Gene
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Long gene name
CD4 molecule
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Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
cardiotrophin 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1995-05-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukin 6 type cytokine family
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
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Identity
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Gene
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Long gene name
leucine zipper protein 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2004-09-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transcription and export complex 1 subunits
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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