Rat Anti-Mouse NKG2-A/C/E-FITC

• Catalog number
  GEN673556
• Price
  Please ask
• Size
  500ug

• Also known as
  NKG2-A/C/E
• Other names
  N/A
• Category
  Secondary Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  Rat IgG2a kappa
• Clone
  20d5
• Host organism
  Rat (Rattus norvegicus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Mouse NKG2 (Mr ~ 43 kDa) The monoclonal antibody 20d5 reacts with an epitope common to the NKG2A, NKG2C and NKG2E isoforms of the CD94/NKG2 heterodimer, a member of the c-type lectin family of inhibitory receptors. Mouse CD94/NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa- 1b.2, 3 NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells. Two-color immunofluorescent staining with the 20d5 antibody reveals two distinct NKG2+ phenotypes within the NK1.1+CD3- spleen cell population: NKG2hi (50%) and NKG2low (50%). With the exception of DBA/2J mice, NKG2 is expressed on NK1.1+ cells from all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).1; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  Fluorescein (FITC) Conjugate
• Concentration
  N/A
• Storage and shipping
  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
• Tested applications
  Flow Cytometry
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

• Gene target
  NKG2-A/C/E  
• Gene symbol
  KLRC1, KLRC4, KLRC3, HLA-A, KLRC2, IGHV5-10-1, CIDEA, RPA1, CDC37P1
• Short name
  Anti-Mouse NKG2-A/C/E-FITC
• Technique
  anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
• Host
  Rat
• Label
  FITC
• Species
  Mouse, Mouses, Rats
• Alternative name
  Anti-Rat Anti-Mouse NKG2-A/C/E-FITC
• Alternative technique
  murine, antibodies, fluorescine

Gene info

• Identity
  HGNC:6374
• Gene
  KLRC1
• Long gene name
  killer cell lectin like receptor C1
• Synonyms gene
  • NKG2
• Synonyms gene name
  • killer cell lectin-like receptor subfamily C, member 1
• Synonyms
  • NKG2-A
  • NKG2-B
  • CD159a
• Synonyms name
  • NKG2-1/B activating NK receptor
• GenBank acession
  • U54782
• Locus
  12p13
• Discovery year
  1998-01-16
• Entrez gene record
  3821
• Pubmed identfication
  • 9598306
• RefSeq identity
  • NM_002259
• Classification
  • C-type lectin domain containing
  • CD molecules
  • Killer cell lectin like receptors
• VEGA ID
  OTTHUMG00000168584

Gene info

• Identity
  HGNC:6377
• Gene
  KLRC4
• Long gene name
  killer cell lectin like receptor C4
• Synonyms gene name
  • killer cell lectin-like receptor subfamily C, member 4
• Synonyms
  • NKG2-F
• GenBank acession
  • U96846
• Locus
  12p13.2
• Discovery year
  1998-09-03
• Entrez gene record
  8302
• Pubmed identfication
  • 9598306
• RefSeq identity
  • NM_013431
• Classification
  • Killer cell lectin like receptors
• VEGA ID
  OTTHUMG00000168575

Gene info

• Identity
  HGNC:6376
• Gene
  KLRC3
• Long gene name
  killer cell lectin like receptor C3
• Synonyms gene name
  • killer cell lectin-like receptor subfamily C, member 3
• Synonyms
  • NKG2-E
• GenBank acession
  • L14542
• Locus
  12p13.2
• Discovery year
  1998-01-16
• Entrez gene record
  3823
• Pubmed identfication
  • 9598306
• RefSeq identity
  • NM_002261
• Classification
  • C-type lectin domain containing
  • Killer cell lectin like receptors
• VEGA ID
  OTTHUMG00000167149

Gene info

• Identity
  HGNC:4931
• Gene
  HLA-A
• Long gene name
  major histocompatibility complex, class I, A
• GenBank acession
  • D32129
• Locus
  6p22.1
• Discovery year
  2001-06-22
• Entrez gene record
  3105
• Pubmed identfication
  • 8838351
• RefSeq identity
  • NM_002116
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000130501
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:6375
• Gene
  KLRC2
• Long gene name
  killer cell lectin like receptor C2
• Synonyms gene name
  • killer cell lectin-like receptor subfamily C, member 2
• Synonyms
  • NKG2-C
  • CD159c
  • NKG2C
• Synonyms name
  • NKG2-C type II integral membrane protein
  • natural killer cell receptor G2-C
• GenBank acession
  • X54869
• Locus
  12p13.2
• Discovery year
  1998-01-16
• Entrez gene record
  3822
• Pubmed identfication
  • 9598306
• RefSeq identity
  • NM_002260
• Classification
  • Killer cell lectin like receptors
  • C-type lectin domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000168532

Gene info

• Identity
  HGNC:5661
• Gene
  IGHV5-10-1
• Long gene name
  immunoglobulin heavy variable 5-10-1
• Synonyms gene
  • IGHV5-A
• Synonyms gene name
  • immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
  • immunoglobulin heavy variable 5-A (provisional)
• Synonyms
  • IGHV5-a
• GenBank acession
  • X92227
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28386
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000191143

Gene info

• Identity
  HGNC:1976
• Gene
  CIDEA
• Long gene name
  cell death inducing DFFA like effector a
• Synonyms gene name
  • cell death-inducing DFFA-like effector a
• Synonyms
  • CIDE-A
• GenBank acession
  • AF041378
• Locus
  18p11.21
• Discovery year
  1998-07-15
• Entrez gene record
  1149
• Pubmed identfication
  • 9564035
  • 18509062
• RefSeq identity
  • NM_001279
• VEGA ID
  OTTHUMG00000131691

Gene info

• Identity
  HGNC:10289
• Gene
  RPA1
• Long gene name
  replication protein A1
• Synonyms gene name
  • replication protein A1 (70kD)
  • replication protein A1, 70kDa
• Synonyms
  • REPA1
  • RPA70
  • HSSB
  • RF-A
  • RP-A
• GenBank acession
  • M63488
• Locus
  17p13.3
• Discovery year
  1993-12-14
• Entrez gene record
  6117
• Pubmed identfication
  • 8454588
• RefSeq identity
  • NM_002945
• Classification
  • Nucleotide excision repair
• VEGA ID
  OTTHUMG00000090579

Gene info

• Identity
  HGNC:34406
• Gene
  CDC37P1
• Long gene name
  cell division cycle 37 pseudogene 1
• Synonyms gene name
  • cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
• Synonyms
  • A-761H5.2
• Locus
  16p11.2
• Discovery year
  2009-01-29
• Entrez gene record
  390688
• Pubmed identfication
  • 15616553
• RefSeq identity
  • NG_009463
• VEGA ID
  OTTHUMG00000175739

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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