Recombinant Human ECE1 Protein (His tag) 20µg

• Catalog number
  PKSH031372-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  ECE;RP3-329E20.1
• Activity
  NA
• Sequence
  Gln 90-Trp 770
• Fusion tag
  N-His
• Accession
  P42892-1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  80 kDa
• AP Mol Mass
  125 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Reconstitution
  Please refer to it for detailed information.
• Background
  Endothelin-converting enzyme 1, also known as ECE-1, is a single-pass type I I membrane protein which belongs to the peptidase M13 family. ECE-1 converts big endothelin-1 to endothelin-1. ECE-1 is a membrane metalloprotease that generates endothelin from its direct precursor big endothelin. Four isoforms of ECE-1 are produced from a single gene through the use of alternate promoters. These isoforms share the same extracellular catalytic domain and contain unique cytosolic tails, which results in their specific subcellular targeting.All isoforms of ECE-1 are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C of ECE-1 are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C of ECE-1 in liver, testis and small intestine; isoform B, C and D of ECE-1 in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Defects in ECE1 are a cause of Hirschsprung disease, cardiac defects and autonomic dysfunction. It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ECE1   Protein   His   tag   20µg  
• Gene symbol
  ECE1, ECE1-AS1
• Short name
  Recombinant ECE1 Protein (His tag) 20µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens endothelin converting enzyme 1 Protein (histidine detection labelled) 20µg
• Alternative technique
  rec
• Alternative to gene target
  endothelin converting enzyme 1, ECE, ECE1 and IDBG-93094 and ENSG00000117298 and 1889, metal ion binding, Cell surfaces, Ece1 and IDBG-199213 and ENSMUSG00000057530 and 230857, ECE1 and IDBG-645619 and ENSBTAG00000002977 and 281133

Gene info

• Identity
  HGNC:3146
• Gene
  ECE1
• Long gene name
  endothelin converting enzyme 1
• Synonyms gene
  • ECE
• GenBank acession
  • D49471
• Locus
  1p36.12
• Discovery year
  1995-12-08
• Entrez gene record
  1889
• Pubmed identfication
  • 7805846
  • 7864876
  • 17592116
• RefSeq identity
  • NM_001397
• Classification
  • M13 metallopeptidases
• VEGA ID
  OTTHUMG00000002625

Gene info

• Identity
  HGNC:40203
• Gene
  ECE1-AS1
• Long gene name
  ECE1 antisense RNA 1
• Locus
  1p36.12
• Discovery year
  2019-07-25
• Entrez gene record
  100506801
• RefSeq identity
  • NR_038404
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products