Dihydrofolate Reductase Activity Kit (Colorimetric)

• Catalog number
  K246-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Absorbance (OD= 340 nm) • Application- Detection of Dihydrofolate Reductase activity
• Detection Method
  Absorbance (OD=340 nm)
• Species Reactivity
  Mammalian
• Applications
  Detection of Dihydrofolate Reductase activity
• Sample Type
  Purified recombinant protein Cell and tissue lysate
• Features Benefits
  • Rapid, sensitive and convenient • The kit measures total Activity of DHFR Activity with limit of quantification 4 mU/ml using recombinant DHFR
• Storage Conditions
  -80°C
• Shipping Conditions
  dry ice
• Shelf life
  12 months
• Background
  Dihydrofolate Reductase (DHFR; 5,6,7,8-tetrahydrofolate NADP oxidoreductase; EC 1.5.1.3), is a ubiquitous enzyme that is present in all eukaryotic and prokaryotic cells. It catalyzes the reduction of dihydrofolate (FH2) to tetrahydrofolate (FH4) using NADPH as a cofactor. FH4 is essential for a number of enzymes that are necessary for the de novo synthesis of purines, thymidylic acid and some amino acids. Inactivation of the DHFR enzymatic activity causes reduction of the intracellular level of FH4, inhibition of RNA and DNA synthesis, and cell death. For this reason, DHFR has been a critically important enzyme as a molecular target in drug discovery. Biovision’s Dihydrofolate Reductase assay kit is based on the ability of DHFR to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. Our assay has been optimized to be carried out in a 96- well plate. The assay is simple, sensitive and can detect as low as 4 mU/ml in a variety of samples.
• Data Sheet Link
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Dihydrofolate   Reductase   Activity   Kit  
• Short name
  Dihydrofolate Reductase Activity Kit (Colorimetric)
• Technique
  colorimetric, colorimetry
• Alternative name
  Dihydrofolate Reductase Activity reagent (colorimetry)
• Alternative technique
  assays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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