HMG-CoA Reductase Catalytic Domain (GST Tagged), human recombinant
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Catalog number7841-100
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PricePlease ask
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Size100 ug
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Synonyms3-Hydroxy-3-methylglutaryl-CoA reductase, HMGR, HMGCR
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Alternative_names3-Hydroxy-3-methylglutaryl-CoA reductase, HMGR, HMGCR
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Description≥90% Pure Human recombinant GST Tagged HMG-CoA Reductase Catalytic Domain, a primary target of statins.
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Activity Specifications test methodThe specific activity was determined to be > 1.0 Unit/mg by BioVision’s HMG-CoA reductase Activity/Inhibitor Screening Kit (Cat # K588).
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Results> 1.0 Unit/mg
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Unit DefinitionOne unit is defined as the amount of enzyme required to convert 1.0 umole of NADPH to NADP+ per minute at 37 °C
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Molecular Weight75.9 kDa (426–888 aa, NT GST Tag)
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Storage Temp-80°C
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Shippingdry ice
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from proprietary buffer containing BSA
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Reconstitution InstructionsBriefly spin down the vial and reconstitute in 50% glycerol in PBS to 0.5-1.0 mg/ml and store at –80°C.
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Background InformationHMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is a transmembrane protein involved in the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. Since HMG-CoA reductase catalyzes the rate limiting step in cholesterol synthesis, it is a primary target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol.
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Amino acid sequence426–888 aa
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMGST3
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Short nameHMG-CoA Reductase Catalytic Domain (GST Tagged), recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHMG-CoA Reductase Catalytic Domain (GST Tagged), H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data