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Antibody Target
Alkaline Phosphatase
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Target Alternative Name
Alkaline phosphatase, germ cell; Alkaline phosphatase, testicular and thymus, Alkaline phosphatase liver/bone/kidney, Alkaline phosphatase, tissue-nonspecific, ALPG, ALPL, Germ cell alkaline phosphatase, Testicular and thymus alkaline phosphatase, Tissue non-specific alkaline phosphatase, Tissue nonspecific ALP (TNAP or TNSALP)
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Antibody Type
Monoclonal
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Species Reactivities
Human
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Immunogen Sequence
Recombinant human ALPL protein
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Accession
P05186
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Gene Id
249
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In 10 mM PBS with 0.05% BSA & 0.05% azide
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Purification
Protein A/G purification
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Application
FC, IF, IHC
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Positive Control
IHC: human endometrial carcinoma, human testicular carcinoma, human ovarian carcinoma, human colon carcinoma
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Application And Usages
FC: 0.5-1 ug/1X106 cells in 0.1 ml, IF: 0.5-1 ug/ml, IHC: 1:50-1:100
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Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Not Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
ALPL
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Short name
Anti-Alkaline Phosphatase Antibody (ALPL/597)
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Isotype
Mouse IgG1, kappa
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Label
Alkaline Phosphatase
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Alternative name
Antibody toAlkaline Phosphatase (antibody to-) (alkaline phosphatase, liver/bone/kidney/597)
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Alternative technique
antibodies
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Alternative to gene target
alkaline phosphatase, liver/bone/kidney, AP-TNAP and APTNAP and HOPS and TNAP and TNSALP, ALPL and IDBG-93194 and ENSG00000162551 and 249, metal ion binding, Extracellular, Alpl and IDBG-199139 and ENSMUSG00000028766 and 11647, ALPL and IDBG-645614 and ENSBTAG00000008951 and 280994
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