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Enhanced Green Fluorescent Protein EGFP

Enhanced Green Fluorescent Protein EGFP is available 3 times from Biovision labs

4999-1000 | Enhanced Green Fluorescent Protein (EGFP) size: 1 mg | 2,801.85 USD

Catalog number 4999-1000
Supplier Biovision
Price 2,801.85 USD
Size 1 mg
Shipping gel pack
Background Information The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP (Gene Bank Accession no. U57607) is a 32.7 kDa monomer with 293 amino acids tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody (Cat.# 3998-100) and GFP antibody (Cat.# 3999-100) on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria (Chalfie et al, 1994), with Ex/Em = 488/507 nm (e489 nm = 55,000 M-1 Cm-1; e280 nm = 21980 M-1 Cm-1).
1. Gene info
Identity 38821
Gene LY6G6F-LY6G6D
Long gene name LY6G6F-LY6G6D readthrough
Synonyms
  • MEGT1
  • MEGT-1
  • G6F-LY6G6D
Synonyms name
  • megakaryocyte-enhanced gene transcript 1 protein
Locus 6p21.33
Discovery year 2019-10-23
Entrez gene record 110599563
Pubmed identfication
  • 18817541
Product images
    #0#.jpegEnhanced Green Fluorescent Protein (EGFP)

4999-5000 | Enhanced Green Fluorescent Protein (EGFP) size: 5 mg | 8,156.80 USD

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4999-100 | Enhanced Green Fluorescent Protein (EGFP) size: 100 ug | 454.72 USD

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Synonyms Recombinant EGFP Protein, Enhanced Green Fluorescent Protein
Alternative_names E-GFP, Enhanced Green Fluorescent Protein
Description Fluorescent protein ideal for subcellular labeling/visualization
Recombinant Yes
Source E. coli
Purity by SDS-PAGE ≥97%
Assay SDS-PAGE
Purity ≥97%
Molecular Weight 32.7 kDa
Storage Temp. -20°C
Shelf Life 12 months
Appearance Lyophilized protein
Physical form description Freeze Dried
Reconstitution Instructions Reconstitute with PBS to 1 mg/ml
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Conjugate The Green Fluorescent Protein (eGFP) of Enhanced Protein ( ) can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
Additional description Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
Gene targetEnhanced Protein
Short name Enhanced Protein ( )
Label green
Alternative name Enhanced Green Fluorescent Protein (EGFP)
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Enhanced Protein ( ) | Technique alternative | 01012195891
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  • Enhanced
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