Enhanced Green Fluorescent Protein (EGFP)
-
Catalog number4999-1000
-
PricePlease ask
-
Size1 mg
-
-
SynonymsRecombinant EGFP Protein, Enhanced Green Fluorescent Protein
-
Alternative_namesE-GFP, Enhanced Green Fluorescent Protein
-
DescriptionFluorescent protein ideal for subcellular labeling/visualization
-
RecombinantYes
-
SourceE. coli
-
Purity by SDS PAGE≥97%
-
AssaySDS-PAGE
-
Purity≥97%
-
Molecular Weight32.7 kDa
-
Storage Temp-20°C
-
Shippinggel pack
-
Shelf Life12 months
-
AppearanceLyophilized protein
-
Physical form descriptionFreeze Dried
-
Reconstitution InstructionsReconstitute with PBS to 1 mg/ml
-
Background InformationThe recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP (Gene Bank Accession no. U57607) is a 32.7 kDa monomer with 293 amino acids tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody (Cat.# 3998-100) and GFP antibody (Cat.# 3999-100) on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria (Chalfie et al, 1994), with Ex/Em = 488/507 nm (e489 nm = 55,000 M-1 Cm-1; e280 nm = 21980 M-1 Cm-1).
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
ConjugateThe Green Fluorescent Protein (eGFP) of Enhanced Protein ( ) can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
-
Additional descriptionFluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
-
Gene target
-
Gene symbolLY6G6F-LY6G6D, ERI3, PPP1R14C
-
Short nameEnhanced Protein ( )
-
Labelgreen
-
Alternative nameEnhanced Green Fluorescent Protein (EGFP)
-
Gene info
-
Identity
-
Gene
-
Long gene nameLY6G6F-LY6G6D readthrough
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year2019-10-23
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nameERI1 exoribonuclease family member 3
-
Synonyms gene
-
Synonyms gene name
- prion protein interacting protein
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-12-13
-
Entrez gene record
-
RefSeq identity
-
Classification
- Exonucleases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameprotein phosphatase 1 regulatory inhibitor subunit 14C
-
Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 14C
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-06-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Protein phosphatase 1 regulatory subunits
-
VEGA ID
