Synonyms
Recombinant EGFP Protein, Enhanced Green Fluorescent Protein
Alternative_names
E-GFP, Enhanced Green Fluorescent Protein
Description
Fluorescent protein ideal for subcellular labeling/visualization
Recombinant
Yes
Source
E. coli
Purity by SDS-PAGE
≥97%
Assay
SDS-PAGE
Purity
≥97%
Molecular Weight
32.7 kDa
Storage Temp.
-20°C
Shelf Life
12 months
Appearance
Lyophilized protein
Physical form description
Freeze Dried
Reconstitution Instructions
Reconstitute with PBS to 1 mg/ml
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Conjugate
The Green Fluorescent Protein (eGFP) of Enhanced Protein ( ) can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
Additional description
Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
Gene targetEnhanced
Protein
Short name
Enhanced Protein ( )
Label
green
Alternative name
Enhanced Green Fluorescent Protein (EGFP)
