Goat Anti-Rabbit IgG (H&L) FITC
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Catalog number
6925-100
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Price
Please ask
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Size
100 µl
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Antibody Target
Rabbit IgG
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Target Alternative Name
Anti-rabbit IgG, FITC-Linked Antibody, FITC linked secondary antibody
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Antibody Type
Secondary antibody
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Species Reactivities
Rabbit
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Immunogen Sequence
Rabbit IgG (H&L)
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In 0.01M Sodium phosphate, 0.25 M NaCl, 1% BSA, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.
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Purification
Affinity Purified
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Application
ELISA, WB, IH, IC.
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Application And Usages
ELISA: 1:20000 – 1:400000, WB: 1:20000 – 1:400000, IH: 1:500 – 1:5000, IC: 1:500 – 1:5000.
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Description
Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) FITC.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
6 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Not Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus, Oryctolagus cuniculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene target
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Gene symbol
CALD1, FUT1, L3MBTL2
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Short name
Goat Anti-Rabbit IgG (H L) FITC
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Technique
Rabbit, anti-, anti, FITC, Goat, IgG, antibody to, antibodies, Fluorescein, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
Rabbit IgG, Heavy & Light chains, IgG
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Label
FITC
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Species
Goats
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Alternative name
caprine Antibody tohost: rabbit Immunoglobulin G (heavy + light chain) fluorecein
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Alternative technique
rabbit-anti, antibodies, caprine, fluorescine, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
caldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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