Rabbit anti Goat IgG H + L FITC
#
-
Catalog number43R-1058
-
Price:
-
Size2 mg
-
-
CategorySecondary Antibody
-
Antibody SubtypeConjugated Secondary Antibodies
-
Area of researchImmunology
-
Type of ImmunogenPurified Goat IgG (H&L)
-
Raised inRabbit
-
SpecificityThis antibody reacts with heavy chains on Goat IgG and light chains on all Goat immunoglobulins.
-
Cross ReactivityNo reactivity is observed to non-immunoglobulin Goat serum proteins.
-
Conjugate TagFITC
-
Grade PurityGrater than 95% pure
-
Method of PurificationAffinity purified using solid phase Goat IgG (H&L)
-
SourceRabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
-
Concentration1.0 mg/ml (lot specific)
-
Form BufferClear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
-
StorageStore at 2-8 deg C.
-
Shipping conditionsBlue Ice
-
Tested forFC; IF; IHC
-
Usage RecommendationsThe optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
-
ConjugationAnti-FITC Antibody
-
Latin nameCapra aegagrus hircus, Oryctolagus cuniculus
-
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Gene target-
-
Gene symbolCALD1, FUT1, L3MBTL2
-
Short nameRabbit anti Goat IgG H + L FITC
-
TechniqueRabbit, anti, FITC, Goat, IgG, antibody to, Fluorescein, goats, IgGs
-
HostRabbit, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
-
IsotypeIgG, IgG
-
LabelFITC
-
SpeciesGoats
-
Alternative nameRabbit anti Goat IgG (H + L) secondary antibody (FITC)
-
Alternative techniquerabbit-anti, antibodies, caprine, fluorescine, immunoglobulins
-
Gene info
-
Identity
-
Gene
-
Long gene namecaldesmon 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1992-07-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namefucosyltransferase 1 (H blood group)
-
Synonyms gene
-
Synonyms gene name
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
-
Synonyms name
-
Locus
-
Discovery year1989-06-06
-
Entrez gene record
-
RefSeq identity
-
Classification
- Fucosyltransferases
- Blood group antigens
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameL3MBTL histone methyl-lysine binding protein 2
-
Synonyms gene name
- l(3)mbt-like 2 (Drosophila)
- L3MBTL2, polycomb repressive complex 1 subunit
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2002-04-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- MBT domain containing
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data