Anti-Rat Macrophage, FITC, (Polyclonal) (rabbit IgG)
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Catalog number
MBS524264
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Price
Please ask
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Size
2 mg
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Products_type
Antibody
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Products_short_name
[Macrophage]
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Products_name_syn
[FITC Adsorbed Rabbit anti-Rat Macrophage]
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Clonality
Polyclonal
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Reactivity
Rat
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Specificity
Macrophage
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Form
FITC 2 mg in phosphate buffered saline
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Concentration
2 mg/ml
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Storage_stability
Stable at 4 degree C. For long term, divide into useful aliquots and store at -20 degree C.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
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Gene target
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Gene symbol
MPEG1, MST1L, MMP12
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Short name
Anti- Macrophage, FITC, (Polyclonal) (rabbit IgG)
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Technique
Polyclonal, Rabbit, anti-, anti, FITC, IgG, antibody to, antibodies, Fluorescein, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Label
FITC
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Species
Rat, Rats
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Alternative name
Antibody toRat Macrophage, fluorecein, (polyclonal) (production species: rabbit Immunoglobulin G)
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Alternative technique
polyclonals, rabbit-anti, antibodies, fluorescine, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
macrophage expressed 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2008-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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