Reverse Transcriptase (cDNA up to 8 kb) Enzyme
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Catalog number
abx071024
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Price
Please ask
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Size
10 kU
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Category
Enzymes
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Clonality
N/A
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Raised in
N/A
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Target
Reverse Transcriptase (cDNA up to 8 kb
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Immunogen
see included datasheet or contact us
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Species reactivity
Human, Mouse, Rat (for other species please inquire)
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Purification
Affinity purified
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Applications
WB (for other applications please contact us)
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Stock availability
Shipped within 5-10 working days.
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Storage conditions
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
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Footnote
This product is for research use only.
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Description
this 1 cDNA is double stranded complementary DNA derived by Abbexa from mRNA with very high fidelity reverse transcription using a pfu or pfx mix, Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional. Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Goup
reverse transcription
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Gene target
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Short name
Reverse Transcriptase (cDNA up 8 kb) Enzyme
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Technique
reverse, Enzyme, cDNA, cDNAs, enzymes
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Label
Unconjugated
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Alternative name
Reverse Transcriptase (complementary Desoxyribonucleic acid up to 8 kb) Enzyme
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Alternative technique
transctription, enzymes, pcr
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Tissue
cdna
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MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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