Reverse Transcriptase (cDNA up to 12 kb) Enzyme

• Catalog number
  abx071025
• Price
  Please ask
• Size
  5x10 kU

• Category
  Enzymes
• Clonality
  N/A
• Raised in
  N/A
• Target
  Reverse Transcriptase (cDNA up to 12 kb
• Immunogen
  see included datasheet or contact us
• Species reactivity
  Human, Mouse, Rat (for other species please inquire)
• Purification
  Affinity purified
• Applications
  WB (for other applications please contact us)
• Stock availability
  Shipped within 5-10 working days.
• Storage conditions
  Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
• Footnote
  This product is for research use only.
• Other products
• Description
  this 1 cDNA is double stranded complementary DNA derived by Abbexa from mRNA with very high fidelity reverse transcription using a pfu or pfx mix, Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional. Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
• Goup
  reverse transcription

• Gene target
  Transcriptase  
• Short name
  Reverse Transcriptase (cDNA up 12 kb) Enzyme
• Technique
  reverse, Enzyme, cDNA, cDNAs, enzymes
• Label
  Unconjugated
• Alternative name
  Reverse Transcriptase (complementary Desoxyribonucleic acid up to 12 kb) Enzyme
• Alternative technique
  transctription, enzymes, pcr
• Tissue
  cdna

MeSH Data

• Name
  Reverse Transcriptase Polymerase Chain Reaction
• Concept
  Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
• Tree numbers
  • E05.393.620.500.725
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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