pECMV-Crh-m-FLAG Plasmid
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Catalog numberPVT15109
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Price348.4 USD
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Size2 ug
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PropertiesAn anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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KitPlasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Short namepECMV-Crh-m- Plasmid
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Techniqueplasmid, plasmids in 1
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LabelFlag
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Alternative namepECMV-corticotropin releasing hormone-m-FLAG Plasmid
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Alternative techniqueplasmids
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Alternative to gene targetcorticotropin releasing hormone, CRF, CRH and IDBG-23777 and ENSG00000147571 and 1392, corticotropin-releasing hormone receptor 2 binding, Extracellular, Crh and IDBG-132673 and ENSMUSG00000049796 and 12918, CRH and IDBG-644607 and ENSBTAG00000033128 and 101910126,280755
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