Mouse Anti-Rat GluN1/NR1 Monoclonal IgG1, Clone S308-48

• Catalog number
  SMC-410D-PCP
• Price
  Please ask
• Size
  100 µg

• Clone
  S308-48
• Immunogen
  Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1
• Antibody s full description
  Mouse Anti-Rat GluN1 Monoclonal IgG1 Antibody, Clone: S308-48: PerCP
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  NMDAR1 Antibody, NMDA Receptor 1 Antibody, NMDAR Antibody, NMDA1 Antibody, GRIN1 Antibody, Glutamate [NMDA] receptor subunit zeta-1 Antibody, Glutamate receptor ionotropic N methyl D aspartate 1 Antibody, MRD8 Antibody, N methyl D aspartate receptor Antibody, N methyl D aspartate receptor channel subunit zeta 1 Antibody, N methyl D aspartate receptor subunit NR1 Antibody, NMD-R1 Antibody, NMDA 1 Antibody, NMDA R1 Antibody, NMDZ1_HUMAN Antibody, NR1 Antibody, GluN1 Antibody
• Raised in
  Mouse
• Antibody s target
  GluN1
• Primary research fields
  Cell Signaling, Neuroscience, Neurotransmitter Receptors, Glutamate Receptors, NMDA Receptors
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~105kDa.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-410 was sufficient for detection of NR1 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  The NMDA receptor (NMDAR), a glutamate receptor, is the predominant molecular device for controlling synaptic plasticity and memory function (1). The NMDA receptor forms a heterotetramer between two NR1 and two NR2 subunits (the subunits are also called glutamate-binding NMDA receptor subunits or GluN for short); two obligatory NR1 subunits and two regionally localized NR2 subunits. A related gene family of NR3 A and B subunits have an inhibitory effect on receptor activity. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits.
• Bibliography
  1. Li F., and Tsien J.Z. (2009) New England J. Medicine. 361: 302-303. 2. Garcia-Gallo M., Renart J., Diaz-Guerra M. (2001) Biochem J. 356: 539-547. 3. Atlason P.T., Garside M.L., Meddows E., Whiting P., McIllhinney R.A.J. (2007) J Biol Chem. 282(35): 25299-25307.
• Released date
  16-May-2012
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_058706.1
• Gene number
  24408
• Protein number
  P35439
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN1/NR1 Monoclonal Antibody, Clone S308-48 (SMC-410). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody (SMC-410) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GluN1/NR1 Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone S308-48 (SMC-410). Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody (SMC-410) at 1:1000. Mouse Anti-GluN1/NR1 Antibody [S308-48] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-410) Mouse Anti-NMDAR1 NMDA receptor Antibody [S308-48] used in Western Blot (WB) on Rat brain membrane lysate (SMC-410)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  GluN1/NR1   Monoclonal   IgG1   S308-48  
• Gene symbol
  SNORD115-48, IGLV5-48, IGHV3-48, NDOR1, RNU6-48P, UTP18, HAUS8, SNORD115-45
• Short name
  Mouse Anti- GluN1/NR1 Monoclonal IgG1, Clone S308-48
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  PerCP
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat GluN1/NR1 monoclonal IgG1, clonality S308-48
• Alternative technique
  murine, antibodies
• Clone name
  S308-48

Gene info

• Identity
  HGNC:33343
• Gene
  SNORD115-48
• Long gene name
  small nucleolar RNA, C/D box 115-48
• Synonyms
  • HBII-52-48
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-16
• Entrez gene record
  100033822
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003362.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5925
• Gene
  IGLV5-48
• Long gene name
  immunoglobulin lambda variable 5-48 (non-functional)
• Synonyms gene name
  • immunoglobulin lambda variable 5-48
• GenBank acession
  • Z73649
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28780
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151041

Gene info

• Identity
  HGNC:5606
• Gene
  IGHV3-48
• Long gene name
  immunoglobulin heavy variable 3-48
• GenBank acession
  • M99675
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28424
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151959

Gene info

• Identity
  HGNC:29838
• Gene
  NDOR1
• Long gene name
  NADPH dependent diflavin oxidoreductase 1
• Synonyms
  • NR1
  • bA350O14.9
  • CIAE1
• Synonyms name
  • NADPH dependent FMN and FAD containing oxidoreductase
• GenBank acession
  • BC015735
• Locus
  9q34.3
• Discovery year
  2004-05-17
• Entrez gene record
  27158
• Pubmed identfication
  • 10625700
  • 12631275
• RefSeq identity
  • NM_014434
• Classification
  • Cytosolic iron-sulfur assembly components
• VEGA ID
  OTTHUMG00000020986

Gene info

• Identity
  HGNC:34292
• Gene
  RNU6-48P
• Long gene name
  RNA, U6 small nuclear 48, pseudogene
• Synonyms gene
  • RNU6-48
• Synonyms gene name
  • RNA, U6 small nuclear 48
• Locus
  1p36.11
• Discovery year
  2008-06-10
• Entrez gene record
  100887744
• RefSeq identity
  • NR_049779

Gene info

• Identity
  HGNC:24274
• Gene
  UTP18
• Long gene name
  UTP18 small subunit processome component
• Synonyms gene
  • WDR50
• Synonyms gene name
  • WD repeat domain 50
  • UTP18 small subunit (SSU) processome component homolog (yeast)
• Synonyms
  • CGI-48
• GenBank acession
  • AF151806
• Locus
  17q21.33
• Discovery year
  2005-02-01
• Entrez gene record
  51096
• Pubmed identfication
  • 10810093
  • 8619474
  • 15590835
• RefSeq identity
  • NM_016001
• Classification
  • UTPb subcomplex
  • WD repeat domain containing
• VEGA ID
  OTTHUMG00000162370

Gene info

• Identity
  HGNC:30532
• Gene
  HAUS8
• Long gene name
  HAUS augmin like complex subunit 8
• Synonyms gene
  • HICE1
• Synonyms gene name
  • HEC1/NDC80 interacting, centrosome associated 1
• Synonyms
  • MGC20533
  • NY-SAR-48
• GenBank acession
  • BC004398
• Locus
  19p13.11
• Discovery year
  2008-12-16
• Entrez gene record
  93323
• Pubmed identfication
  • 19427217
• RefSeq identity
  • NM_001011699
• Classification
  • HAUS augmin like complex
• VEGA ID
  OTTHUMG00000182672

Gene info

• Identity
  HGNC:33064
• Gene
  SNORD115-45
• Long gene name
  small nucleolar RNA, C/D box 115-45
• Synonyms
  • HBII-52-45
  • SNORD115-48
• Locus
  15q11.2
• Discovery year
  2011-08-22
• Entrez gene record
  100036566
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003498
• Classification
  • Small nucleolar RNAs, C/D box

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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