Human Recombinant GSTA1

• Catalog number
  6346-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Human Recombinant GSTA1
• Alternative_names
  Glutathione S-transferase alpha 1, GST2, GSTA1-1, GTH1.
• Description
  Plays a role in detoxification of electrophilic compounds
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is 5-8 units/mg.
• Unit Definition
  Specific activity is defined as the amount of enzyme that conjugates 1.0 µ mole of 1-chloro-2, 4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
• Molecular Weight
  25.6 kDa
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.
• Background Information
  Glutathione S-transferase A1 in humans is encoded by the GSTA1 gene. Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. GSTA1 protects the cells from reactive oxygen species and is highly expressed in liver.
• Amino acid sequence
  MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GSTA1  
• Gene symbol
  GSTA1
• Short name
  Recombinant GSTA1
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. GSTA1
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4626
• Gene
  GSTA1
• Long gene name
  glutathione S-transferase alpha 1
• Synonyms gene name
  • glutathione S-transferase A1
• Locus
  6p12.2
• Discovery year
  1991-12-06
• Entrez gene record
  2938
• Pubmed identfication
  • 9503014
• Classification
  • Soluble glutathione S-transferases
• VEGA ID
  OTTHUMG00000014861

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products