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Category
Proteins
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Antibody Subtype
Recombinant Proteins
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Area of research
Proteases, Inhibitors, & Enzymes
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Reactivity
Human
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Residues
1-222 amino acids: MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 90% pure
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Concentration
1 mg/ml (lot specific)
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Form Buffer
Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 1 mM DTT and 10% glycerol.
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Storage
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE
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Bioactivity
Specific activity is 5-8 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 DEG C.
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Assay Information
1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant GSTA1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.
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Additional Information
GSTA1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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