Goat, Goat anti-biotin, FITC conjugated
-
Catalog number
AS10 1453
-
Price
Please ask
-
Size
1 mg
-
-
Available ordering format
Liquid
-
Immunogen
Biotin
-
Raised in
Goat
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Affinity purified IgG
-
How to reconstitute
See included datasheet or contact our support service
-
Storage condition
2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
-
Verified applications
Contact Gentaur support center
-
Connected products
other secondary antibodies against biotin
-
Recommended dilutions for use
This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2000 for most applications.
-
Molecular weight expected аpparent
see datasheet
-
Verified reactivity
to be determined
-
Possible reactivity
to be determined
-
No reactivity
to be determined
-
Supplementary information
-
References
Refer to PubMed
-
Scientific context
Goat anti-biotin is a secondary antibody conjugated to FITC, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
-
Notes
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
-
Protein number
Refer to NCBI
-
TAIR number
Refer to NCBI
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
-
Conjugation
Biotinylated, Anti-FITC Antibody
-
Latin name
Capra aegagrus hircus
-
Gene target
-
Short name
Goat, Goat anti- , FITC conjugated
-
Technique
anti-, anti, FITC, Goat, antibody to, antibodies, antibody Conjugates, Fluorescein, goats
-
Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
Label
Biotin
-
Species
Goats
-
Alternative name
caprine, caprine Antibody tobiotinilated, fluorecein coupled
-
Alternative technique
antibodies, caprine, fluorescine
-
MeSH Data
-
Name
-
Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products