Hrk Antibody

• Catalog number
  3176-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Hrk
• Target Alternative Name
  BID3
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Synthetic peptide corresponding to residues surrounding amino acids 50 of human HRK
• Accession
  AAC34931
• Gene Id
  8739
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) antigen affinity purified rabbit anti-human Hrk polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blot analysis (1 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects human Hrk fused with green fluorescent protein in crude extracts of E. coli transfected with Hrk-GFP.
• Description
  Members of the Bcl-2 family of proteins interact to regulate programmed cell death, or apoptosis. Various homodimers and heterodimers formed by proteins in this family can either promote or inhibit apoptosis. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect on certain hematopoietic cell lines following growth factor withdrawal. Additional apoptotic inhibitors in this family include Bcl-x, Bcl-w, Mcl-1, Bag-1 and A1. Pro-apoptotic members of this family include Bax, Bad, Bak, NBK (Bik), BID and Hrk. Hrk (for harakiri), designated DP5 or neuronal death protein in mouse and rat, contains a BH3 domain with high homology to other Bcl-2 family members but lacks the conserved BH1 and BH2 domains. Physical interaction of Hrk with Bcl-2 or Bcl-xL inhibits the apoptotic activity of Hrk.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Hrk  
• Gene symbol
  HRK
• Short name
  Hrk Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  harakiri, BCL2 interacting protein (contains only BH3 domain) (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  harakiri, BCL2 interacting protein (contains only BH3 domain), DP5 and HARAKIRI, HRK and IDBG-59297 and ENSG00000135116 and 8739, protein binding, Plasma membranes, Hrk and IDBG-195140 and ENSMUSG00000046607 and 12123, HRK and IDBG-633660 and ENSBTAG00000047484 and 787139

Gene info

• Identity
  HGNC:5185
• Gene
  HRK
• Long gene name
  harakiri, BCL2 interacting protein
• Synonyms gene name
  • harakiri, BCL2-interacting protein (contains only BH3 domain)
• Synonyms
  • DP5
• Synonyms name
  • death protein 5
• GenBank acession
  • U76376
• Locus
  12q24.22
• Discovery year
  1999-05-21
• Entrez gene record
  8739
• Pubmed identfication
  • 9130713
• Classification
  • BCL2 homology region 3 (BH3) only
• VEGA ID
  OTTHUMG00000169328

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products