-
Synonyms
LDLR, FH, FHC, LDLCQ2, Low-Density Lipoprotein (LDL) Receptor
-
Alternative_names
LDLR, FH, FHC, LDLCQ2, Low-Density Lipoprotein (LDL) Receptor
-
Description
Mediates the endocytosis of cholesterol-rich LDL
-
Recombinant
Yes
-
Source
HEK293 cells
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Biological activity
Measured in a competitive binding assay. When human LDL is immobilized at 1 ug/ml (100 ul/well), recombinant human LDLR inhibits 50% binding of biotinylated recombinant human LDLR (0.5 ug/ml) at the concentration range of 0.35 – 2.5 ug/ml.
-
Results
Measured in a competitive binding assay. When human LDL is immobilized at 1 ug/ml (100 ul/well), recombinant human LDLR inhibits 50% binding of biotinylated recombinant human LDLR (0.5 ug/ml) at the concentration range of 0.35 – 2.5 ug/ml.
-
Molecular Weight
This protein is fused with a C-terminal 6×his tag and has a calculated MW of 86 kDa. The predicted N-terminal is Ala22 or Asp193. Corresponding to the mature and immature form, DTT-reduced protein migrates as 95-110 kDa &125-140 kDa polypeptide in SDS-PAGE resulting from different glycosylation.
-
Storage Temp
-20°C
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Appearance
Lyophilized
-
Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
-
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
-
Background Information
Low-Density Lipoprotein (LDL) Receptor, also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
LDLR, LDLR-AS1
-
Short name
CellExp™ LDLR, recombinant
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Species
Humans
-
Alternative name
H. sapiens CellExp™ low density lipoprotein receptor, H. sapiens Rec.
-
Alternative technique
rec
-
Alternative to gene target
low density lipoprotein receptor, FH and FHC and LDLCQ2, LDLR and IDBG-28808 and ENSG00000130164 and 3949, very-low-density lipoprotein particle receptor activity, Cell surfaces, Ldlr and IDBG-141358 and ENSMUSG00000032193 and 16835, LDLR and IDBG-643476 and ENSBTAG00000012314 and 281276
-
