Goat anti Bovine IgG HRP
-
Catalog number43C-CB0141
-
PricePlease ask
-
Size2 mg
-
-
CategorySecondary Antibody
-
Antibody SubtypeConjugated Secondary Antibodies
-
Area of researchImmunology
-
Type of ImmunogenGoat anti-bovine IgG (HRP) was raised in goat using bovine IgG F(ab')2 fragment ..
-
Raised inGoat
-
SpecificityBovine IgG
-
Cross ReactivityShould be determined by the end user
-
Conjugate TagHRP
-
Method of PurificationGoat anti Bovine IgG (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
-
Form BufferLyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% gentamicin sulfate. DO NOT add NaN3.
-
StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
-
Shipping conditionsBlue Ice
-
Tested forELISA; IHC; WB
-
Usage RecommendationsELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:10,000
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Latin nameCapra aegagrus hircus
-
PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
-
ConjugationHRP Conjugated
-
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
-
Short nameGoat anti IgG
-
Techniqueanti, Goat, IgG, antibody to, goats, IgGs
-
HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
IsotypeIgG, IgG
-
LabelHRP
-
SpeciesBovine, Bos Taurus genes are available form the Bovine Genome database. Goats
-
Alternative nameGoat anti Bovine IgG secondary antibody (HRP)
-
Alternative techniqueantibodies, caprine, immunoglobulins
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data