Glutathione (GSH/GSSG/Total) Fluorometric Assay Kit
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Catalog number
K264-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Fluorescence (Ex/Em 340/420 nm) • Species reactivity- Mammalian • Applications- The kit provides unique formula and buffers and procedures for detecting the GSH, GSSG, and total glutathione individually
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Sample Type
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes only ~1-2 hours • Fast and convenient • The assay is easy to perform and detects 2-400 ng/µl of GSH, GSSG or total glutathione.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Glutathione is the major intracellular low-molecular-weight thiol that plays a critical role in cellular defense against oxidative stress in tissues and cells. Commercially available glutathione detection kits, such as the DTNB-enzyme cycling glutathione assay kit or the Monochlorobimane based assay kit hardly distinguish between reduced glutathione (GSH; FW: 307) and oxidized glutathione (GSSG; FW: 612). BioVision’s Glutathione Detection Kit provides a unique, convenient tool for detecting GSH, GSSG, and total glutathione individually. In the assay, OPA, reacts with GSH (not GSSG), generating fluorescence, so GSH can be specifically quantified. Adding a reducing agent converts GSSG to GSH, so (GSH + GSSG) can be determined. To measure GSSG specifically, a GSH Quencher is added to remove GSH, preventing reaction with OPA (while GSSG is unaffected). Reducing agent is then added to destroy excess quencher and to convert GSSG to GSH. Thus, GSSG can be specifically quantified. The kit provides a unique procedure and buffer formula to eliminate protein thiol interference and to stabilize GSH and GSSG in solution.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
GSX1
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Short name
Glutathione (GSH/GSSG/ ) Fluorometric Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
GSH (GSH/GSSG/Total) fluorescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
total
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Gene info
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Identity
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Gene
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Long gene name
GS homeobox 1
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year
2003-02-18
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
Product images
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