OxiSelect™ Total Glutathione (GSSG/GSH) Assay test kit
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Catalog number
STA-312
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Price
Please ask
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Size
100 assays
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We send this reagant on
Blue Ice
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The temperatureerature for storage is
Upon receipt, store the NADPH at -80ºC. Prepare single use aliquots and avoid multiple freeze/thaw cycles. Store the remaining test kit components at 4ºC.
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Technical file
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SDS or safty data sheet
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Type of reagent
Oxidative Stress/Damage
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Related techniques
Oxidative Stress/ Damage, Antioxidant Assays, Glutathione Assay, Glutathione Assay
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Use of the reagent
The OxiSelect™ Total Glutathione Assay test kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG). Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH. Subsequently, the chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm. The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve. The rate of chromophore production is proportional to the concentration of glutathione within the sample. The rate can be determined from the absorbance change over time. Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.
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Cellular use
Measures total glutathione (oxidized and reduced)
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Protocol information
Sensitive detection as low as 4 nM
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Reagent properties
Suitable for use with serum, plasma, saliva, urine, tissue extracts, and mammalian or plant cell lysates
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Living modified organisme in this reagent or LMO
Not applicable
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,39
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Test
Cellular biology labs supplies other types of Assays as 1.
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Gene target
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Gene symbol
GSX1
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Short name
OxiSelect™ Glutathione (GSSG/GSH) Assay test kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
OxiSelect™ Total GSH (GSSG/GSH) test test reagent
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Alternative technique
arrays, tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
total
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Gene info
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Identity
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Gene
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Long gene name
GS homeobox 1
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year
2003-02-18
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
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