Mouse Monoclonal IgG1 Anti-p16 INK4a (DCS-50) [DCS-50]
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Catalog numberP16002
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PricePlease ask
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Size50 µg
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AvailabilityPlease contact us to check the availability
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ImmunogenRecombinant human p16 INK4a molecule
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SpecificityHuman p16 protein
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Species reactivityHuman, other species not tested
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Product presentationProtein A affinity purified antibody lyophilized from PBS pH7.4 with BSA and Na-Azide 0.09%
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG1
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Antibody s cloneDCS-50
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Product is supplied aslyophilisate
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Recommended concentration for use1:10
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Protein concentration50 µg/ml after reconstitution in 1 ml distilled water
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Positive controlColon carcinoma, HPV-pos. cervical carcinoma
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PretreatmentPre-treatment with Unmasking Fluid C (Citrate Buffer, Art. No. DE000) or Unmasking Fluid G (Art. No. DE007) at 96-100 degree of Celsius is recommended for paraffin sections.
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Tested applicationsIHC (C,P), IP
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Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage methodStore at 2-8°C
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UniProt numberP42771 (CD2A1_HUMAN)
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Litterature1. Koh J., Enders G.H., Dynlacht B.D., and Harlow E. (1995) Tumour-derived p16 allels encoding proteins defective in cell cycle inhibition. Nature 375; 506-510. 2. Reed A.L., Califano J., Cairns P., Westra W.H., Jones R.M., et al. (1996) High frequency of p16 (CDKN2/MTS-1/INK4A) inactivation of head and neck squamous cell carcinoma. Cancer Res. 56(16); 3630-3633. 3. Geradts J., Hruban R.H., Schütte M., Kern S.E., and Maynard R. (2000) Immunohistochemical p16INK4a analysis of archival tumors with deletion, hypermethylation, or mutation of the CDKN2/MTS1 gene. A comparison of four commercial antibodies. Appl. Immun 4. Negri G., Egarter-Vigl E., Kasal A., Romano F., Haitel A., Mian C. (2003) p16INK4a is a useful marker for the diagnosis of adenocarcinoma of the cervix uteri and its precursors: an immunohistochemical study with immunocytochemical correlations. Am J. S 5. Kotaro R. Shibata,Tomoki Aoyama,Yasuko Shima et al. (2007) Expression of the p16INK4A Gene Is Associated Closely with Senescence of Human Mesenchymal Stem Cells and Is Potentially Silenced by DNA Methylation During In Vitro Expansion Stemm Cells vol. 9; 2371–2382.
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGLV1-50, IGHV3-50, DCTN2, GAP43, RNU6-50P, CENPU, RNVU1-4, WIPI2
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Short nameBioprime
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TechniqueMouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG1
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SpeciesMouse, Mouses
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Alternative namep16 INK4a (DCS-50)
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 1-50 (non-functional)
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Synonyms gene name
- immunoglobulin lambda variable 1-50
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-50 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-50
- immunoglobulin heavy variable 3-50 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedynactin subunit 2
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Synonyms gene name
- dynactin 2 (p50)
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-10-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Dynactin subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegrowth associated protein 43
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Synonyms
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Synonyms name
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Locus
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Discovery year1990-07-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 50, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear pseudogene 1
- RNA, U6 small nuclear 50
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namecentromere protein U
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Synonyms gene
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Synonyms gene name
- MLF1 interacting protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Constitutive centromere associated network
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 4
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Synonyms gene
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Synonyms gene name
- RNA, variant U1 small nuclear 5
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Synonyms
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Locus
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Discovery year2013-04-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene nameWD repeat domain, phosphoinositide interacting 2
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Synonyms
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Locus
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Discovery year2005-11-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WIPI family
- Autophagy related
- WD repeat domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
