Isotype Control Ms IgG1/IgG1-FITC/RPE
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Catalog number
MG1G1MCS100H
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Price
Please ask
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Size
100 tests
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Product type
Antibody
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Clone
HybIgG1/HybIgG1
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Format
FITC/RPE
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Size 2
1ml
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Target Species
Irrelevant
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Reg
CE-IVD
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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Properties
This AcZon Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. AcZon FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Conjugation
Anti-FITC Antibody
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Group
isotypes
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Gene target
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Gene symbol
RPE
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Short name
Isotype Control Ms IgG1/IgG1-FITC/RPE
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Technique
Control, Isotype, FITC, controls, Fluorescein, Isotypes and controls
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Isotype
Mouse IgG1/IgG1, IgG1
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Label
FITC
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Alternative name
isoform reference Ms IgG1/IgG1-fluorecein/RPE
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Alternative technique
control, controls, fluorescine
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Tissue
control
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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