Rabbit F(ab')2 Anti-Sheep IgG (H+L), Human SP ads-AP

• Catalog number
  MBS674788
• Price
  Please ask
• Size
  1 mL

• Products_type
  Secondary Antibody
• Products_short_name
  [Rabbit F(ab')2 Anti-Sheep IgG (H+L)]
• Products_name_syn
  [Rabbit F(ab')2 Anti-Sheep IgG(H+L), Human SP ads- Alkaline Phosphatase-Labeled; Rabbit F(ab')2 Anti-Sheep IgG (H+L) (Absorbed against human serum proteins)]
• Clonality
  Polyclonal
• Reactivity
  Sheep
• Specificity
  Reacts with the heavy and light chains of sheep IgG as demonstrated by ELISA. May also react with the light chains of other goat immunoglobulins. Minimal cross reactivity with human immunoglobulins.
• Purity
  Affinity chromatography on sheep IgG covalently coupled to agarose. Gel filtration chromatography of pepsin digested antibody.
• Form
  Alkaline Phosphatase (AP) Conjugate
• Storage_stability
  The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
• Tested_application
  ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  f(ab')2s
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  H+L   ads-AP  
• Gene symbol
  JUND, TFAP2A, TFAP2E, PTPRCAP, AP5B1, TFAP2B, KLF12, TRRAP, PHYHIP, TFAP2D
• Short name
  Rabbit F(ab')2 Anti- IgG (H+L), SP ads-AP
• Technique
  F(ab')2, Rabbit, anti-, anti, IgG, antibody to, antibodies,  F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. IgGs
• Host
  Rabbit, Rabbits
• Isotype
  IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
• Label
  AP
• Species
  Human, Humans, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  production species: rabbit F(Antibody')2 Antibody toSheep Immunoglobulin G (heavy + light chain), H. sapiens SP ads-AP
• Alternative technique
  secondairy, rabbit-anti, antibodies, immunoglobulins

Gene info

• Identity
  HGNC:6206
• Gene
  JUND
• Long gene name
  JunD proto-oncogene, AP-1 transcription factor subunit
• Synonyms
  • AP-1
• Synonyms name
  • transcription factor jun-D
  • JunD-FL isoform
  • activator protein 1
• Locus
  19p13.11
• Discovery year
  1990-09-10
• Entrez gene record
  3727
• Pubmed identfication
  • 2112242
  • 1903194
• RefSeq identity
  • NM_005354
• Classification
  • Basic leucine zipper proteins
  • Jun transcription factor family
• VEGA ID
  OTTHUMG00000183355

Gene info

• Identity
  HGNC:11742
• Gene
  TFAP2A
• Long gene name
  transcription factor AP-2 alpha
• Synonyms gene
  • TFAP2
  • AP2TF
• Synonyms gene name
  • transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha)
  • transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
• Synonyms
  • AP-2
  • AP-2alpha
• GenBank acession
  • X52611
• Locus
  6p24.3
• Discovery year
  1991-09-12
• Entrez gene record
  7020
• Pubmed identfication
  • 1916817
  • 3063603
• RefSeq identity
  • NM_003220
• Classification
  • Transcription factor AP-2 family
• VEGA ID
  OTTHUMG00000014235

Gene info

• Identity
  HGNC:30774
• Gene
  TFAP2E
• Long gene name
  transcription factor AP-2 epsilon
• Synonyms gene name
  • transcription factor AP-2 epsilon (activating enhancer binding protein 2 epsilon)
• Synonyms
  • AP2E
  • AP-2epsilon
• GenBank acession
  • BC041175
• Locus
  1p34.3
• Discovery year
  2004-11-05
• Entrez gene record
  339488
• Pubmed identfication
  • 14636996
• RefSeq identity
  • NM_178548
• Classification
  • Transcription factor AP-2 family
• VEGA ID
  OTTHUMG00000004388

Gene info

• Identity
  HGNC:9667
• Gene
  PTPRCAP
• Long gene name
  protein tyrosine phosphatase receptor type C associated protein
• Synonyms
  • LPAP
  • CD45-AP
• Locus
  11q13.2
• Discovery year
  1996-04-22
• Entrez gene record
  5790
• RefSeq identity
  • NM_005608
• VEGA ID
  OTTHUMG00000150325

Gene info

• Identity
  HGNC:25104
• Gene
  AP5B1
• Long gene name
  adaptor related protein complex 5 subunit beta 1
• Synonyms gene name
  • adaptor-related protein complex 5, beta 1 subunit
  • adaptor related protein complex 5 beta 1 subunit
• Synonyms
  • PP1030
  • AP-5
  • DKFZp761E198
• GenBank acession
  • JQ313135
• Locus
  11q13.1
• Discovery year
  2012-02-27
• Entrez gene record
  91056
• Pubmed identfication
  • 22022230
• RefSeq identity
  • NM_138368
• Classification
  • Adaptor related protein complex 5
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000166593

Gene info

• Identity
  HGNC:11743
• Gene
  TFAP2B
• Long gene name
  transcription factor AP-2 beta
• Synonyms gene name
  • transcription factor AP-2 beta (activating enhancer-binding protein 2 beta)
  • transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
• Synonyms
  • AP2-B
  • AP-2beta
• GenBank acession
  • X95694
• Locus
  6p12.3
• Discovery year
  1995-12-18
• Entrez gene record
  7021
• Pubmed identfication
  • 7555706
  • 8661133
• RefSeq identity
  • NM_003221
• Classification
  • Transcription factor AP-2 family
• VEGA ID
  OTTHUMG00000014836

Gene info

• Identity
  HGNC:6346
• Gene
  KLF12
• Long gene name
  Kruppel like factor 12
• Synonyms
  • AP-2rep
  • HSPC122
  • AP2REP
• Synonyms name
  • KLF12 zinc finger transcriptional repressor
  • AP-2rep transcription factor
  • AP-2 repressor
• GenBank acession
  • AJ243274
• Locus
  13q22.1
• Discovery year
  2000-05-25
• Entrez gene record
  11278
• Pubmed identfication
  • 10704285
• RefSeq identity
  • NM_007249
• Classification
  • Zinc fingers C2H2-type
  • Kruppel like factors
• VEGA ID
  OTTHUMG00000017078

Gene info

• Identity
  HGNC:12347
• Gene
  TRRAP
• Long gene name
  transformation/transcription domain associated protein
• Synonyms gene name
  • transformation/transcription domain-associated protein
• Synonyms
  • TR-AP
  • PAF400
  • Tra1
• GenBank acession
  • AF076974
• Locus
  7q22.1
• Discovery year
  1998-10-06
• Entrez gene record
  8295
• Pubmed identfication
  • 9708738
  • 9885574
• RefSeq identity
  • NM_003496
• Classification
  • MicroRNA protein coding host genes
  • SAGA complex
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000150403

Gene info

• Identity
  HGNC:16865
• Gene
  PHYHIP
• Long gene name
  phytanoyl-CoA 2-hydroxylase interacting protein
• Synonyms gene
  • DYRK1AP3
• Synonyms gene name
  • phytanoyl-CoA hydroxylase interacting protein
  • DYRK1A interacting protein 3
• Synonyms
  • KIAA0273
  • PAHX-AP
• GenBank acession
  • D87463
• Locus
  8p21.3
• Discovery year
  2001-11-16
• Entrez gene record
  9796
• Pubmed identfication
  • 9039502
  • 10686344
• RefSeq identity
  • NM_014759
• VEGA ID
  OTTHUMG00000163776

Gene info

• Identity
  HGNC:15581
• Gene
  TFAP2D
• Long gene name
  transcription factor AP-2 delta
• Synonyms gene
  • TFAP2BL1
• Synonyms gene name
  • transcription factor AP-2 beta (activating enhancer-binding protein 2 beta)-like 1
  • transcription factor AP-2 delta (activating enhancer binding protein 2 delta)
• Synonyms
  • AP-2delta
• GenBank acession
  • AY028376
• Locus
  6p12.3
• Discovery year
  2001-04-27
• Entrez gene record
  83741
• Pubmed identfication
  • 11733187
• RefSeq identity
  • NM_172238
• Classification
  • Transcription factor AP-2 family

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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