Calpain-2 Antibody

• Catalog number
  3372-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  Calpain 2
• Target Alternative Name
  M-Calpain , Calpain 2 , Calpian II , CANPL2 , CANP2 , millimolar-calpain , mCANP , FLJ39928 , CANPml , EC 3.4.22.53
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide mapping to the C-terminus of the domain III of human Calpain 2 large subunit
• Accession
  NP_001739
• Gene Id
  824
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  100 µl affinity purified rabbit polyclonal anti-calpain 2 antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (1:200-1000 dilutions). However, the optimal concentrations should be determined individually. The anti-calpain 2 antibody recognizes the 80 kDa Calpain 2. It does not cross-react with other calpains
• Description
  Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF-hand domain IV. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Calpain-2  
• Gene symbol
  CAPN8, CAPN2, CAPNS2
• Short name
  Calpain-2 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Calpain-2 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:1485
• Gene
  CAPN8
• Long gene name
  calpain 8
• Synonyms gene name
  • calpain 8 (nCL-2)
• Synonyms
  • nCL-2
• Locus
  1q41
• Discovery year
  1998-09-25
• Entrez gene record
  388743
• Pubmed identfication
  • 7690035
  • 8889549
• RefSeq identity
  • NM_001143962
• Classification
  • Calpains
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000037378

Gene info

• Identity
  HGNC:1479
• Gene
  CAPN2
• Long gene name
  calpain 2
• Synonyms gene name
  • calpain 2, (m/II) large subunit
• Synonyms
  • mCANP
  • CANPml
  • CANPL2
• GenBank acession
  • J04700
• Locus
  1q41
• Discovery year
  1989-06-30
• Entrez gene record
  824
• Pubmed identfication
  • 2852952
  • 2539381
• RefSeq identity
  • NM_001748
• Classification
  • EF-hand domain containing
  • Calpains
• VEGA ID
  OTTHUMG00000037376

Gene info

• Identity
  HGNC:16371
• Gene
  CAPNS2
• Long gene name
  calpain small subunit 2
• Synonyms
  • MGC12536
  • MGC14804
• GenBank acession
  • AY052551
• Locus
  16q12.2
• Discovery year
  2004-07-19
• Entrez gene record
  84290
• Pubmed identfication
  • 11853546
• RefSeq identity
  • NM_032330
• Classification
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000167812

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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