Single Stranded DNA Binding Protein, E. coli
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Catalog number9220-50
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PricePlease ask
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Size50 ug
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SynonymsSSB,Single- Stranded DNA Binding Protein, E. coli
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Alternative_namesSSB,Single- Stranded DNA Binding Protein, E. coli
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DescriptionA homo-tetrameric protein, which binds onto single stranded DNA to coordinate replication, repair, and recombinatio
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Recombinant1
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SourceE. coli strain containing an overproducing clone of E. coli SSB protein.
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Purity by SDS PAGE≥ 99% by SEC and SDS-PAGE analysis
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AssayThe activity of SSB protein was determined using Gel Shift Assay. Varying amounts of SSB (150 ng to 86 ug) were able to bind onto 22 fmol of M13mp18 ssDNA in a 20 ul reaction volume and affect ssDNA mobility. Sample was loaded using 10x Orange DNA Loading Buffer (BV Cat# 2110-10) onto a 1% agarose gel. Gel was electrophoresed at 3 V/cm for 5 hr.
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Molecular Weight18.9 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life24 months
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ConcentrationLot specific
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AppearanceLiquid
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Physical form descriptionLiquid in 50 mM Tris pH 8.0 and 50% Glycerol
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Background InformationSingle Stranded DNA Binding Protein (SSB) is a homo-tetrameric protein, which binds onto single stranded DNA (ssDNA) to coordinate replication, repair, and recombination. It possesses a N-terminal DNA binding core and a C-terminal protein-protein interacting tail. ssDNA can form secondary structures and SSB prevents their formations. SSB can be added to PCR and DNA sequencing reactions to enhance the DNA polymerases processivity by allowing them to access their substrate more easily.
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Amino acid sequenceFull-length protein
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Gene target
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Gene symbolPRKDC, HLA-DOA
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Short nameSingle Stranded DNA Binding Protein,
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Techniquedna
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Hoste. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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SpeciesE. coli, E. coli
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Alternative nameSingle Stranded Desoxyribonucleic acid Binding Protein, E. coli
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Alternative techniqueescherichia
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Gene info
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Identity
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Gene
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Long gene nameprotein kinase, DNA-activated, catalytic subunit
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Synonyms gene
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Synonyms gene name
- protein kinase, DNA-activated, catalytic polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DO alpha
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases