Single Stranded DNA Binding Protein, E. coli

  • Catalog number
    9220-100
  • Price
    Please ask
  • Size
    100 ug
  • Synonyms
    SSB,Single- Stranded DNA Binding Protein, E. coli
  • Alternative_names
    SSB,Single- Stranded DNA Binding Protein, E. coli
  • Description
    A homo-tetrameric protein, which binds onto single stranded DNA to coordinate replication, repair, and recombinatio
  • Recombinant
    1
  • Source
    E. coli strain containing an overproducing clone of E. coli SSB protein.
  • Purity by SDS PAGE
    ≥ 99% by SEC and SDS-PAGE analysis
  • Assay
    The activity of SSB protein was determined using Gel Shift Assay. Varying amounts of SSB (150 ng to 86 ug) were able to bind onto 22 fmol of M13mp18 ssDNA in a 20 ul reaction volume and affect ssDNA mobility. Sample was loaded using 10x Orange DNA Loading Buffer (BV Cat# 2110-10) onto a 1% agarose gel. Gel was electrophoresed at 3 V/cm for 5 hr.
  • Molecular Weight
    18.9 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    24 months
  • Concentration
    Lot specific
  • Appearance
    Liquid
  • Physical form description
    Liquid in 50 mM Tris pH 8.0 and 50% Glycerol
  • Background Information
    Single Stranded DNA Binding Protein (SSB) is a homo-tetrameric protein, which binds onto single stranded DNA (ssDNA) to coordinate replication, repair, and recombination. It possesses a N-terminal DNA binding core and a C-terminal protein-protein interacting tail. ssDNA can form secondary structures and SSB prevents their formations. SSB can be added to PCR and DNA sequencing reactions to enhance the DNA polymerases processivity by allowing them to access their substrate more easily.
  • Amino acid sequence
    Full-length protein
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Gene target
    Single   Stranded   DNA   Binding   Protein  
  • Gene symbol
    PRKDC, HLA-DOA
  • Short name
    Single Stranded DNA Binding Protein,
  • Technique
    dna
  • Host
    e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
  • Species
    E. coli, E. coli
  • Alternative name
    Single Stranded Desoxyribonucleic acid Binding Protein, E. coli
  • Alternative technique
    escherichia
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