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Products_type
Secondary Antibody
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Products_short_name
[IgA (alpha chain)]
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Products_name_syn
[Goat anti-Human IgA (alpha chain) - Affinity Pure]
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Other_names
[IgA, partial]
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Reactivity
Human. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins light chains on all human immunoglobulins
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Specificity
Based on IEP, this antibody reacts with: heavy (alpha) chains on Human IgA
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Human IgA.
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Form
Clear, fluorescent yellow liquid
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Concentration
1.50 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
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Test
A high affinity purification column was use to purify Goat anti- IgA (alpha chain) - Pure, FITC Conjugate by MyBioSource by chromatographic size exclusion. MyBioSource supplies antibodies that are for research of human proteins.
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Description
The Goat anti- IgA (alpha chain) - Pure, FITC Conjugate is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti- IgA (alpha chain) - Pure, FITC Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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Gene target
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Short name
Goat anti- IgA (alpha chain) - Pure, FITC Conjugate
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Technique
anti-Human, affinity, anti-, anti, FITC, Goat, Pure, IgA, antibody to, antibodies, antibodies against human proteins, Fluorescein, goats, IgAs, pure products
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgA, IgA
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Label
FITC
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Species
Human, Goats, Humans
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Alternative name
caprine Antibody toHuman Immunoglobulin A (a epitope) - protein Pure, fluorecein Conjugate
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Alternative technique
antibodies, purified, caprine, fluorescine, immunoglobulin-a
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