Anti-Protein C Tag Antibody
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Catalog number
A1287-50
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Price
Please ask
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Size
50 µg
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Antibody Target
Protein C
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Target Alternative Name
HPC4, Protein C
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Antibody Type
Monoclonal
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Clone
2H12F4
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Immunogen Sequence
Protein C tag peptide conjugated to KLH
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide
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Purification
Affinity purified
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Application
WB, IP, FC, ICC/IF, ELISA
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Positive Control
WB: Protein C tagged protein transfected CHO cell lysates, IF: Protein C tagged protein transfected CHO cell lysates
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Application And Usages
WB: 0.1-0.5 µg/ml, IP: 1-5 µg/ml, FC: 1-3 µg for 1 x 106 cells, ICC/IF: 1-3 µg/ml, ELISA: 0.001-0.002 µg/ml.
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Description
Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Protein C Tag is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein. Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins. Protein C (HPC4) Tag Antibody, is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with Protein C tag peptide conjugated to KLH.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans
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Handling
The antibody solution should be gently mixed before use
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
Anti-Protein C Tag Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Tags
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Isotype
Mouse IgG1, κ
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Label
tag
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Alternative name
Antibody toProtein C detection labelled (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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