Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit
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Catalog number
K670-100
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Price
Please ask
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Size
100 assays
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Description
Only Kit on the market to measure Triose Phosphate Isomerase (TPI) Activity
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Summary
• Detection method- Absorbance (OD 450 nm) • Applications: - Measurement of TPI activity in various tissues and cells - Analysis of glycolysis & pentose phosphate pathway and lipid metabolism
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Detection Method
Absorbance (OD 450 nm)
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Applications
• Measurement of TPI activity in various tissues and cells • Analysis of glycolysis & pentose phosphate pathway and lipid metabolism
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Sample Type
• Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: Adherent or suspension Cells • Human serum or plasma
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Features Benefits
• Simple, highly sensitive, and high-throughput. • The assay can measure Triose Phosphate Isomerase activity as low as 40 mU/ml.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Triose Phosphate Isomerase (TPI or TIM: EC 5.3.1.1) is an important enzyme for glycolysis. It reversibly interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms. In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment. BioVision’s Triose Phosphate Isomerase Activity Assay kit provides a quick and easy way for monitoring Triose Phosphate Isomerase activity in a variety of samples. In this kit, Triose Phosphate Isomerase converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the Enzyme Mix & Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, & high-throughput and can detect Triose Phosphate Isomerase activity as low as 40 mU/ml.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Triose Phosphate Isomerase (TPI) Activity colorimetry test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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