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Alternative name1
Anti-Mouse Anti-Goat IgG (H&L) FITC
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Alternative name2
Murine Anti-Caprine IgG (H&L) FITC
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Alternative name3
Mus musculus Anti-Caprinae IgG (H&L) FITC
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Alternative name4
IgG (H&L) FITC
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Alternative name5
N/A
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Other names
N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Secondary Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
N/A
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Clone
Polyclonal antibody
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Host organism
Mouse
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Species reactivity
Goat
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Specificity and cross reactivity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
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Purification method
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
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Form Appearance
Liquid in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, containing 1% BSA, 50% glycerol, 0.05% Sodium Azide
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Concentration
N/A
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Storage and shipping
Shipped at Store productone at +4 degrees Celsius.. Upon delivery aliquot and store productone should be stored at -20 degrees Celsius. for six months. Avoid freeze/thaw cycles.
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Tested for
Immunofluorescence (IF), Flow Cytometry (FC/FACS)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus, Mus musculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Gene symbol
CALD1, FUT1, L3MBTL2
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Short name
Mouse Anti-Goat IgG (H L) FITC
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Technique
anti-, Mouse, anti, FITC, Goat, IgG, antibody to, antibodies, Fluorescein, goats, IgGs, mouses
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Host
mouse, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, Heavy & Light chains, IgG
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Label
FITC
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Species
Mouse, Goats, Mouses
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Alternative name
Mouse Antibody toGoat Immunoglobulin G (heavy + light chain) fluorecein
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Alternative technique
murine, antibodies, caprine, fluorescine, immunoglobulins
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